Confirmatory correlation analyses, after Anova?

Dear everybody, I conducted a series of ANOVAs (and MANCOVAs) to verify the presence of differences between two groups for a series of variables. A reviwer commented that " the relationship they...

02 November 2020 4,982 6 View

How to model in Arena Rockwell that all entities must clear their tails at the end of the simulation?

I am modeling the flow of visitors during a working day in a museum. The main problem is that I don't know how to model in Arena Rockwell the fact that all entities must clear the queue at the end...

20 February 2020 9,457 1 View

LC-MS: same compound, 2 RT?

Hi, I have an issues with the detection of a standard on LC-MS. I run MGDG standard (prepared in methanol, LC-MS grade) on HILIC BEH Amide column; the run is 15 minutes long, using a gradient...

16 June 2019 4,355 4 View

How would you morphological gloss truncated words in spontaneous speech in an Italian dialect?

I have a data set of spontaneous speech in Romanesco (Italian dialect spoken in Rome), which I need to gloss morphologically. These are the turns in question (but there are other similar...

20 February 2019 7,567 3 View

Chloroform resistant tips in lipid analysis?

Hi, I'm wondering if someone is using carbon filtered pipette tips for lipid analysis. At the moment I extract lipids from bacterial cultures using methanol:chloroform and I use Hamilton syringe...

22 January 2019 2,829 1 View

Can predictors of a Dependent variable be considered as control variables if those are not the main focus of a study?

Hello, I am carrying out SEM analysis with a model including two Independent variable (A,B) one mediator (C) and two dependent variable (D,E). For reasons of statistical control and incremental...

06 March 2018 8,275 3 View

Do you have an exact reference to know if I have to restart from passage number zero when I thaw cells?

Concerning numeration cells passage, there are two school of thought. One said that we have to restart from zero and one said that we have to continuing the numeration (e.g. freeze at #4, maintain...

19 July 2016 7,135 2 View

No title

I am trying to figure out whether a CO2 (GHG) emission reduction goal of -60% by 2030 is feasible for Switzerland and am happy to have any inputs, reading advice etc. Thanks!

24 June 2014 8,401 3 View

What are the best cell lines to use as control for Casr gene expression using real-time PCR?

We test Casr expression in human stem cells with real-time PCR (Taqman probes, Life technologies), but only using a large amount of DNA (about 250ng) we observed ct at 32-34 cycles. We would...

04 February 2014 3,073 1 View

Do you know a method for phytochelatin extraction by HPLC?

I would like information on a methods for phytochelatins determination with HPLC and without the use of the additional step of derivatization and without MS, if possible.

15 December 2013 8,573 1 View

No title

03 March 2021 5,360 2 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

Does PHA-P stimulate the proliferation of lymphocytes from mice?

Hello, I will start performing MLR using lymphocytes from mice's spleen and I would like to know the better way for the stimulation. If is using PHA-P or CD3/CD28? I would like to know as well if...

23 February 2021 5,476 4 View

Is it okay to mention you distributed your qualitrics study link through social media sites?

Just want to be clear and know it is okay to say I have distributed the qualitrics link through social media such as Facebook, Instagram and Twitter to ensure my target audience is reached in my...

21 February 2021 5,143 2 View

Looking for advices and tips to improve the staining and the gating strategy in human blood?

Hi, I am staining a human blood with CD19, CD3,CD14 and CD16. My gating strategy in FlowJo goes like this: - First, I gate a general population SSC-A VS FSC-A => Then gate for single cells, =>...

21 February 2021 5,724 4 View

How T cells recognise non-peptide antigens?

This might sound very basic question, but please share with me an explanation of how the T cell is activated when the antigen is not a peptide? What happens? And what are the molecules involved in...

21 February 2021 564 3 View

Who are the possible experts I can reach out to regarding the reseach topic: Activated Carbon as Water Filter?

Hi! Me and my team mates are currently new in doing a research study. We do not have any previous experiences in research. And we just wanted to know what type of experts (the field they in) can...

19 February 2021 9,596 4 View

How to locate and collect the pineal gland in the amphibian brain?

Our team has been trying to locate and collect the pineal gland in the American bullfrog's brain (Lithobates catesbeianus) for some time now. However, we don't have a standardized protocol for...

18 February 2021 3,453 4 View

FDC and FRC cell populations in mouse spleen?

I was trying to isolate fibroblastic reticular cells (FRC) and follicular dendritic cells from mice spleen, but kept getting poor yield. I found from literature that the frequency of FDC is around...

17 February 2021 3,506 4 View

Comsol Maximum number of Newton iterations reached?

Comsol is investigating deformation due to gravity. Gravity is applied to the nanosheets to check for displacement and stress. If I input the Young's modulus of the channel to about 1 [GPa], the...

15 February 2021 6,027 3 View