How to model the causal effect of working part-time compared to full-time on health?

Hi there, I have to estimate the causal effect of part-time work compared to full-time work on health. I have an unbalanced panel data set with t=2008-2017, except for year 2009. The main issue...

11 December 2020 3,170 3 View

How to correlate bacteria community data with volatile profiles and with fly community data using ordination techniques?

Carrion Ecology: What is the best way the statistically analyse the correlation of bacteria community data to volatile profiles and to fly community data across 3 time points using ordination...

24 November 2020 5,831 3 View

Does anyone have any advice for Intracellular Staining of methyltransferase for flow cytometry?

Hi All, I have been attempting intracellular staining of a methyltransferase and have been running into issues with consistency. I have attempted sequential staining with a PE secondary and have...

20 November 2020 2,314 1 View

URGENT: How to eliminate mold contamination from brain tissue slices?

03 November 2020 8,357 2 View

How many casual deaths constitute scientific evidence? Do we know the risks of repeating influenza vaccination?

Italy (2014). Around 35 death after and very closer to the flu vaccination Spain (2020). At least 45 death reported in correlation with adyuvate flu vaccination and 6 time risk to died with...

22 October 2020 2,746 2 View

Use of FcR blocking reagent for B cell surface IgG staining in flow cytometry?

I would like to measure cell surface IgG in human culture of B cells. I have culture of human peripheral blood mononuclear cells (PBMCs) that were immortalized with EBV (Epstein-Barr Virus) and...

30 August 2020 6,805 2 View

After incubating overnight in blocking buffer (BSA) at 4C, how long can i incubate in Primary antibody the following day?

Hello Everyone, I usually block my membrane in BSA blocking buffer for 1 hour before incubating in primary overnight. This time i was pressed for time and decided to block at 4C over night....

05 August 2020 7,007 4 View

Request for suggestion on bimonthly journals in industrial water pollution management ?

Can anyone suggest some bimonthly journals which are either Scopus or UGC indexed in the area industrial water pollution management? thank you?

24 July 2020 2,036 2 View

Supplier for NiTi powder?

28 June 2020 6,530 4 View

Which is the most recent phylyogenetic tree for rodents?

Dear all Does someone have a recent rodent phylogenetic tree (preferably in Nexus format or any other format usable in R) or knows a publication with a recent tree? Currently I am still using...

24 May 2020 3,620 4 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View