27 August 2021 1 7K Report

I am looking for a 11BHSD1 specific inhibitor to use in a rat cell culture

More Kasey Belanger's questions See All
Best method for computing intraclass correlations for clinical research study?

I am hoping to compute intraclass correlations to evaluate interrater reliability (IRR) for interviews conduct with participants in a clinical psychology study. Specifically, we want to determine...

26 June 2023 4,501 0 View

How to merge many similar excel files into SPSS using syntax?

I am fairly new to syntax writing/coding and need to merge many excel files (with the same set of variables) into one SPSS dataset but it must be done with syntax. So far, all of the loops for...

31 March 2022 6,635 3 View

Trouble digesting supercoiled plasmid into linear form?

I used the TOPO TA Cloning Kit for Sequencing to clone a desired gene into the pCR®4-TOPO® vector. I have been able to confirm the presence of my gene by colony PCR. I then extracted my plasmids...

28 June 2021 349 5 View

Ellipses versus convex hulls for NMDS - which is better?

Is there a statistical or philosophical argument for choosing between ellipses or convex hulls to highlight differences between groups on an NMDS plot? My guess is that, visually, hulls can...

20 August 2020 1,323 1 View

Brain Flow Cytometry Protocol for Tregs?

Does anyone have a good protocol for flow cytometry on the brain looking at T cell profiles? Specifically Tregs?

19 June 2019 4,896 2 View

Advice for making large batches of media?

I am seeking advice for preparing and dispensing large batches of media for plant tissue culture. Generally I have only prepared 1L at a time in a 2L flask. However I would like to start preparing...

14 August 2018 6,798 6 View

Is there any research here on the topic of depression/suicide among non-custodial fathers?

Hi there, One of the studies in this area must be 'quasi-experimental,' and the other must be a "quantitative" or "mixed methods" research design. Thank you :) Angela

09 April 2018 7,952 3 View

Precipitate with MS media?

When preparing MS media (distilled water, MS + sucrose, agar; adjusted to pH 5.8) I have noticed that, after autoclaving, there is a small amount of precipitate in the flask. It mixes back into...

14 November 2016 8,551 7 View

Approximately how many cells can you expect to recover from buffy coat?

I realize that the number of white blood cells in any given whole blood sample will vary drastically depending on the health of the donor. However, is it possible to calculate a rough average of...

20 November 2014 4,377 2 View

Can anyone suggest a method for statistically measuring gastric epithelial intercellular spacing?

I have used transmission electron microscopy to observe morphological differences among the epithelial cells of the stomach mucosa and have found a difference in the intercellular spacing between...

28 April 2014 1,493 2 View

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Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

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How to Add Missing Water Molecules in Protein-Membrane Simulations?

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Does long-term culture of murine Monocytes result in macrophage differentiation?

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In what part of the brain´s rabbit bdnf is located and include references please?

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