we have been sorting cells from human tumors directly into regular trizol. Compared to trizol LS, regular trizol can tolerate less sample volume. however, I am afraid, some of the samples were frozen in trizol with high sample volume (due to facs sorting, which is the source of the added sample volume), so the ratio of trizol and max tolerated sample volume was not maintained. Is there any way I can rescue these samples to get a good quality and RNA yield?