We want to measure ATP consumption and have performed luciferase / luciferin assay which works fine. However, if there is a cheaper alternative we could as well perform this assay in practical courses.
HPLC would be probably more expensive. Do you have nearby any device able to measure 384 well plates.WIth them you really minimize the expenditure of reagents.
I agree with Rafael that the cost in 384 well plates is not high. You can use the Kinase RR Kit described in 67. Lundin, A. and Eriksson, J. (2008) A real-time bioluminescent HTS method for measuring protein kinase activity influenced neither by ATP concentration nor by luciferase inhibition, Assay Drug Dev Technol 6 (4), 531-541. The price is around 260 EUR for 1000 tests in a reaction volume of 50 µL, which can be reduced.
Contact me at [email protected] and I will provide further details.
Best regards, Arne
If the cost of working time (to produce/optimize) is not an issue, you can just make your own ATP reagent. Firefly luciferase is very cheap if you express/purify it yourself: I was regularly getting >100 mg from just 200 ml E. coli culture. D-luciferin is also quite cheap: 1g can be bought for ~250$ and this will be enough for ages.
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