Hello everyone;
I recently started to work with the oral squamous carcinoma cell line Cal27 to measure cell death against Cisplatin, but the cell death rate is too high under culture condition, between 18% and 27%, and I don´t have much experience with this cells. Do you know how to reduce these levels of cell death?
I used 2 different culture media: IMDM (Iscove's Modification of DMEM) and DMEM high glucose with 4 mM L-Glutamine, both supplemented with 10% FBS, Plasmocin Prophylactic at 2,5 ug/mL and Penicillin/Streptomycin at 100 U/mL. These cells are at 37°C 5% CO2 in incubator.
Hello Felipe González
You can find several important information on ATCC website concerning Cal27 : https://www.atcc.org/products/crl-2095
To decrease the cell mortality, the most important things are to renew the medium every 2-3 days and to use a subcultivation ratio of 1:6
Best of luck
There are several strategies that can be used to reduce cell death rate in Cal27 cells, which are a type of human oral squamous cell carcinoma cell line. Here are a few possible approaches:
It is important to note that the specific approach to reducing cell death in Cal27 cells may depend on the specific experimental design and goals of the study. Consulting with a cell culture expert or reviewing relevant literature can also help determine the best strategies for reducing cell death rate in Cal27 cells.
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