Why and how to avoid negative values in optical density by spectrophotometry when measurements corrected by blank solvent?

I am trying to assess chlorophyll-a content from coral tissue using spectrophotometry (with SPECTROstar Nano, BMG). However, I always seem to obtain some negative values in my optical density raw...

20 January 2021 1,542 4 View

What is the best way to differentiate L. rhamnosus and L. casei in plate co-cultivation?

I'm having troubles in the differentiation and quantitation of each bacteria population during co-cultivation. The morphologic characteristics are very similar and it's really difficult to count...

13 January 2021 1,550 1 View

Is GPD 2021 an official and real event?

I was invited to this conference organized by the Hazel pharmaceutical group in Prague 2021. I want to know if the event is real and if any other collage in the pharmacology/ drug discovery area...

25 December 2020 4,407 3 View

How Important are Professional Organizations to you & What Types?categories of Benefits/interactions are you looking for?

This is a two (2) part question specifically for but Professional please chime in. I humbly believe that no matter what the stage we are all students and constantly learn from each other.

09 December 2020 9,296 4 View

What statistical tests can I conduct for a survey with mostly dichotomous survey questions?

I wanted to know what other statistical tests I can conduct for a mostly dichotomous survey. I am assessing GIS use in graduate public health programs and I am not using a Likert-scale for my...

20 November 2020 1,025 3 View

Biotech Startup Research?

26 October 2020 9,698 5 View

Have you ever included two last authors in the co-authorship of a publication?

Hello, As you must know, it is now sometimes possible to have several co-first authors in the co-authorship of a scientific publication, when two persons led the paper and had equal contribution...

21 September 2020 2,995 5 View

Which is the viscosity recommended for using Methyl cellulose to perform oral gavage (mice )?

Hello, we would like to perform oral gavage in mice. The vehicle recommended for our drug consist on 0.5% methylcellulose and 5% Dimethylacetamide (0.1 ml/ 10g mouse). Could somebody help us to...

20 August 2020 8,720 2 View

Where to find the NMR data from a log file instead of using Gaussian?

I do not have Gaussian at home and for unforeseen circumstances I cannot get to a computer to visualize my NMR spectra. Where do I find this data in my log file for proton NMR?

17 March 2020 3,676 1 View

What software is available to model the impact of design change in complex products?

This question relates to research into the management of engineering design information and the impact of innovation. Complex products such as aircraft, trains and ships are high-value, long-life...

18 December 2019 316 3 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

Reagents for histology slides?

Dear all, I am planning to study histology and want to do that at home. I have only stained a blood smear with Giemsa but now want to try a wider range of animal tissues. What reagents will I...

24 February 2021 5,430 3 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

Is it possible that Cancer cells could survive in heparinized blood for 48 hours?

Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?

23 February 2021 3,840 3 View

Why at farmer level Cow/Buffalo are not done artificial insemination just after 2 month for yearly calving?

Physiologically in cow, oestrous heat again goes on the peak after 2 months but locally this is not observed and AI is after two months but done after at least 6 months.

17 February 2021 685 4 View