Im trying to use it with cow tissue, but I don't know if Im doing the protocol right.
Would you like to try immunohistochemistry on paraffin blocks of the tissue? CD34 and SMA may be helpful.
I want to run a moderated mediated path analysis in Mplus. The model is based similar to PROCESS model 35 expect I want to run it with 6 IVS and 2 DVs simultaneously. I have been searching but...
18 June 2024 3,592 3 View
I am trying to conduct an a priori Monte Carlo power analysis for a multi-group path analysis. Does anyone have a sample syntax for Mplus or a reference? I have collected effect sizes for each of...
14 May 2024 9,795 1 View
Three of my samples have negative percent inhibition while the other three samples have positive percent inhibition. For the negative values, the absorbance of the samples are higher than that of...
06 May 2024 4,439 0 View
I am completing my thesis on the potential moderating effect of parent social media use frequency on parent-child communication and family functioning. Literature has indicated relationships...
13 January 2024 8,070 1 View
Hi, I am currently working through the evaluation process with a team and we are trying to look at the common traits of children with higher level skills and children who are still in the ELL...
03 December 2023 2,263 0 View
I would be interested in knowing the thermal decomposition by thermogravimetric analysis of Labrafac lipophile WL1349 since this item is not reported in the SDS by the supplier. Thanks in advance.
10 November 2023 8,388 0 View
More detail: I'm currently growing K562 cells. I have them shaking at 50 rpm in an incubator supplemented with 5% CO2. However, this morning we discovered that our incubator had run out of CO2...
17 October 2023 2,730 0 View
My question emerges as I have just came across an interesting correlation (non-causal) between Blue Brain's project on 11-D neuronal structures using algebraic topology, and 10-D Calabi-Yau...
08 October 2023 2,718 1 View
Only when hemolytic anemia results in a two- to threefold or larger increase in hemoglobin catabolism does jaundice owing to rise of serum unconjugated bilirubin become noticeable. The level of...
07 September 2023 3,103 0 View
Hi. I am working with placental explants and I want to measure explant viability but using a more faster and cheaper method instead of LDH activity in culture media. I know that MTT and TTC...
16 August 2023 8,393 0 View
Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)
05 August 2024 9,537 2 View
In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...
01 August 2024 4,403 4 View
I want to perform a CRISPRa/i library screening for candidate regulatory factors that affect pathogenic Th17 differentiation in PBMC. But I am wondering how to define the size of gene library, so...
31 July 2024 2,150 0 View
Hi all, My lab has Thermo Scientific™ Invitrogen™ EVOS™ FL Auto 2 Imaging System, and I was wondering if I will be able to use it with whole blood, whilst focusing on platelets? The idea would...
24 July 2024 9,337 3 View
Hello, good morning. I would like to know which plate I can use to infect fresh PBMC with virus and culture for 24 hours. I have tried using 12-well and 24-well plates, but many cells adhere and...
24 July 2024 2,158 2 View
hi every one I am making vector construction (for fusion proteins) and in this moment I wanna to amplification of ADAM17 prodomain with PCR. to yet, I couldn't amplified the ADAM17 prodomain with...
21 July 2024 8,660 1 View
Hi, I need help in detaching adherent monocytes from well plates. I isolated the PBMCs from buffy coat and I did CD14+ positive selection to collect only the monocytes. I let them adhere on my...
20 July 2024 7,922 2 View
Cow dung manure is more powerful than artificial fertilizer.
19 July 2024 7,392 7 View
Is this organic cow dung manure now important than artificial fertilizer.if good then why ?
19 July 2024 9,052 2 View
Sometimes I see the shadow like bands and its not true band. I want to know that what's the reason for it. I am using 2% gel for running genotyping samples I have uploaded the gel picture in both...
19 July 2024 148 6 View