04 July 2024 1 2K Report

I tried to google it but only the JBI website shows anything. I was hoping to see a much more thorough explanation of the 10 questions, but I could not find any. Thank you for your advice.

More Daryl Eborda's questions See All
Solvent recovery after extraction - Separation of Isopropanol + Water - how to do it best?

I want to make some yeast extractions with a protocol that uses Isopropanol and NaOH. So in the end i will have an azeotrope of Isopropanol and Water that comes from the cell and NaOH. Can you...

08 July 2024 9,200 2 View

TLC - Chamber saturation problems - what to do to avoid?

I am trying to use a method from a paper by Do et al (https://www.sciencedirect.com/science/article/abs/pii/S0021967320311043?via%3Dihub) for a plant fingerprint. Short version: what could be...

31 May 2024 3,981 0 View

Resoudre l'equation de Mathieu avec Python(Spyder) ?

Soit l'équation de Mathieu : x¨+[delta+epsilon*cos(t)]x=0 *Ecrire un programme python qui resoud numriquement cette equation pour plusieurs...

13 May 2024 5,797 2 View

Which paper can you recommend for chamber saturation in TLC?

Hi there, adding a paper or towel to the tlc chamber is told to fasten the chamber saturation in TLC. But i could not find any information what paper can best be used for that - which works/which...

24 April 2024 7,495 4 View

Troubleshooting TLC - Plant extract - strange in polar mobile Phase - What could be the reason?

I wanted to create the fingerprint of a phenol rich plant extract (dried powder, 15min ultra sound extraction in methanol) following the paper of Do et al (Complementary developing solvents for...

22 April 2024 10,031 4 View

Membrane protein extraction using hypotonic lysis?

I am working with suspension cells, JY cells in particular. I have been using hypotonic lysis (buffer components: 10 mM Tris-HCl pH 7.4) to attempt to obtain cytosolic and membrane protein...

18 March 2024 6,544 4 View

Contaminated Sample - Influence of mold on yeast / Lipids?

Yeast from a small scale fermentation was heat inactivated and stored in a refrigerator after centrifugation - about 60-70% water content. The Yeast was planned for lipid/sterol extraction. Sadly...

07 February 2024 5,295 5 View

What are some colorectal cancer datasets I can use to compare gene expression of CRC vs normal samples/patients?

Doing research on MSS-CRC and have shortlisted a few targets. Was wondering if there are any public datasets that looks at gene expression of genes in MSS-CRC compared to other types of CRC and...

14 October 2023 1,522 1 View

Recreating experiment : BSA solution + ratio - correct values?

I want to recreate and extend an experiment from a paper and am not sure if my math is corrected. None of the authors is registered here. The authors did the following (text of the paper in...

01 October 2023 5,419 1 View

Is there a whatman filter paper or similar that can be used for binding studies of Bovine serum albumin? (Albumin cant pass, smaller metabolites can)?

Bovine serum albumin has a mass of about 66kDa. I want to do binding studies of Bovine serum albumin with several secondary metabolites with a mass up to 2.5kDa. Can you recommend a filter -...

26 September 2023 3,210 4 View

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