How to dilute Modified Citrus Pectin ( EcoNugenics, Santa Rosa CA, USA) in the water? I have tried different methods such as heating and dissolving with PBS, DMSO or alcohol. But I even could not get 1% MCP solution (0.1 gram MCP in 10 mL water. It was not completely dissolved that you could see sediment at the bottom).
Hello, Mr. Shao. My lab colleague has the same problem with MCP and inulin. Dissolving does not appear to be complete for this Polysaccharides. However, studying these two cases helped to make progress in the work:
https://www.researchgate.net/post/How_can_I_dissolve_PectaSol-C_modified_citrus_pectin_powder_to_use_it_in_cell_culture
https://www.researchgate.net/post/How_to_properly_dissolve_inulin_as_a_pharmaceutical_ingredient_for_formulation_studies
Reduce the concentration used in work (for example, using 70 mg MCP in 10 ml water). Place the solution with a magnetic stirring bar on a hot plate stirrer at 80° C and raise the pH of the solution using NaOH 2N to dissolve slowly. It takes a long time for the resulting solution to remain stable and less precipitate/gelatinize. Other things worth trying (Of course I'm not sure): Citric acid at 90 °C for 90 min at a ratio of 1:80 (w/v). (?!) Using an ultrasonic bath and Fenton reaction for better mixing.
I am interested to know the opinions and experiences of other researchers in the face of MCP solubility.
- Badges
- Science topic