Too low protein concentration for Western Blot - any advice for polyacrylamide gel method?

Currently have punched mouse brain samples of different regions that are of too low protein concentration for Western Blot. Has anyone used the polyacrylamide gel electrophoresis method to detect...

09 January 2021 3,955 3 View

Western Blot: any reasonable alternatives or troubleshooting advice for punched samples?

Hi, I currently have punched mouse brain tissue separated by brain region (using the cryostat) and the initial plan was to do Western Blot analysis on the punched tissue. We already have the...

01 January 2021 8,884 1 View

Does anyone have experience or suggestions on the polyacrylamide gel method of protein purification and measurement for punched samples?

Hello, I currently have sliced and punched mouse brain tissue using the cryostat and I wish to analyze it for the concentrations of different dopamine receptors. We initially desired to use...

01 January 2021 9,455 2 View

How to insert a line of median into scatter plot in SPSS? Multiple variables possible?

Hi! I am using SPSS chart builder to do grouped scatter plots. In the first scatter, I have two groups on the x-axis. I want to add a line of median to each group for better comparison. How can I...

17 November 2020 8,693 3 View

Economic evaluation in mental health services / DataBASE to use?

Hi! I am conducting a master thesis in the field of economic evaluation and mental health services as systematic literature review. Now I consider what databases to use. I have been thinking to...

09 September 2020 2,010 3 View

Can I use in a meta analysis case reports and rcts? And what calculation Do you raccomend( proportion, relative risk....)?

21 August 2020 5,190 3 View

Where can I find credible statistics for social media platforms?

I wonder where I can find credible statistics about patterns of using social networking sites that can be used as a reference in an academic thesis, preferably downloadable. Thanks

08 May 2020 4,932 5 View

Where can I find research papers or data concerning Demographic and Socioeconomic Characteristics of Immigrants and Refugees ?

Hello Everyone, I do hope that all of you are doing well and staying safe amid the COVID-19. I'm trying to find research papers or data that gives a global view about the difference in the...

03 April 2020 4,157 1 View

Why can't I see collagen in SDS-PAGE?

I try to resolve collagen on SDS-PAGE. My goal is to resolve the collagen extracted from skin scaffolds. I use ThermoFisher Bolt mini gels. I can see the protein ladder migrating and also I can...

15 March 2020 9,906 8 View

Your opinion on pulsed field electrophoresis instruments?

Hello, I am interested in running an experiment requiring pulsed field electrophoresis. The primary samples will be budding yeasts chromosomes and minor set of samples with chromosome sizes up...

30 October 2019 7,068 2 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View