Qurshid Hasan Khan

9 Questions 19 Answers 0 Followers

Questions related from Qurshid Hasan Khan

How to identify conserved miRNA if related data is not available in miRBase?

I have miRNA data of a biotrophic fungal pathogen, I would like to identify conserved miRNA by homology approach, but in miRBase the fungal miRNA data is not available. Kindly provide your...

02 February 2019 5,864 2 View

Small RNA Next Generation Sequencing?

I want to do small RNA Next Generation Sequencing. To publish my paper in a high impact journal, do I need to take at least 2 replicates or pooling the sample without any replicate would work. I...

12 December 2017 6,324 2 View

How to amplify fragment of approximately 2500 bp?

Hi I want to amplify the fragment of 2500 bp, but everytime it failed. I performed touchdown PCR and I got amplicon size of 500 bp. What might be the reason that I am unable to get the desired...

09 September 2016 4,554 7 View

What does number indicates at nodes in phylogenetic analysis?

Hi I recently started working on phylogenetic analysis. I made a tree the picture is attached below. What does number indicates at nodes of the tree. What more inference can I draw from the...

03 March 2016 9,286 13 View

Transporter prediction based on amino acid sequence?

I have an amino acid sequence of 110 which codes for a functional transporter. Utilizing which online tool and what are the possible way by which I can confirm the structure, functioning, or any...

02 February 2016 1,018 3 View

Double bands after restriction digestion?

I got 2 bands after restriction digestion of unknown gene fragment, does it mean that my gene has restriction site apart from vector. Can I assume that addition of these two band will give me the...

01 January 2016 9,863 5 View

Why there is difference in band position in uncut plasmid and restriction digested?

I ran uncut plasmid with my insert and digested, what will be the reason for the difference as the uncut plasmid consist of the insert and the digested plasmid released the insert, so the band...

01 January 2016 5,940 5 View

Is there any need to use a single digested plasmid for a good gel image?

When we use the plasmid with insert, uncut plasmid, double digested plasmid, and ladder for gel pic, why there is a need to use single digested plasmid in making a good gel image.

12 December 2015 1,638 3 View

Can someone help with blue-white screening?

I have been doing TA cloning for quite sometime. My sample is giving me only few white colonies. I took two control, the first control is plasmid with insert, which resulted in full plate white...

08 August 2015 3,437 4 View