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Questions related from Qurshid Hasan Khan
I have miRNA data of a biotrophic fungal pathogen, I would like to identify conserved miRNA by homology approach, but in miRBase the fungal miRNA data is not available. Kindly provide your...
02 February 2019 5,863 2 View
I want to do small RNA Next Generation Sequencing. To publish my paper in a high impact journal, do I need to take at least 2 replicates or pooling the sample without any replicate would work. I...
12 December 2017 6,322 2 View
Hi I want to amplify the fragment of 2500 bp, but everytime it failed. I performed touchdown PCR and I got amplicon size of 500 bp. What might be the reason that I am unable to get the desired...
09 September 2016 4,553 7 View
Hi I recently started working on phylogenetic analysis. I made a tree the picture is attached below. What does number indicates at nodes of the tree. What more inference can I draw from the...
03 March 2016 9,285 13 View
I have an amino acid sequence of 110 which codes for a functional transporter. Utilizing which online tool and what are the possible way by which I can confirm the structure, functioning, or any...
02 February 2016 1,017 3 View
I got 2 bands after restriction digestion of unknown gene fragment, does it mean that my gene has restriction site apart from vector. Can I assume that addition of these two band will give me the...
01 January 2016 9,861 5 View
I ran uncut plasmid with my insert and digested, what will be the reason for the difference as the uncut plasmid consist of the insert and the digested plasmid released the insert, so the band...
01 January 2016 5,938 5 View
When we use the plasmid with insert, uncut plasmid, double digested plasmid, and ladder for gel pic, why there is a need to use single digested plasmid in making a good gel image.
12 December 2015 1,638 3 View
I have been doing TA cloning for quite sometime. My sample is giving me only few white colonies. I took two control, the first control is plasmid with insert, which resulted in full plate white...
08 August 2015 3,434 4 View