How to identify conserved miRNA if related data is not available in miRBase?

I have miRNA data of a biotrophic fungal pathogen, I would like to identify conserved miRNA by homology approach, but in miRBase the fungal miRNA data is not available. Kindly provide your...

01 February 2019 5,796 2 View

Small RNA Next Generation Sequencing?

I want to do small RNA Next Generation Sequencing. To publish my paper in a high impact journal, do I need to take at least 2 replicates or pooling the sample without any replicate would work. I...

11 December 2017 6,227 2 View

How to amplify fragment of approximately 2500 bp?

08 September 2016 4,478 7 View

What does number indicates at nodes in phylogenetic analysis?

02 March 2016 9,211 13 View

Why there is difference in band position in uncut plasmid and restriction digested?

31 December 2015 5,764 5 View

Double bands after restriction digestion?

31 December 2015 9,787 5 View

Is there any need to use a single digested plasmid for a good gel image?

When we use the plasmid with insert, uncut plasmid, double digested plasmid, and ladder for gel pic, why there is a need to use single digested plasmid in making a good gel image.

11 December 2015 1,537 3 View

Can someone help with blue-white screening?

I have been doing TA cloning for quite sometime. My sample is giving me only few white colonies. I took two control, the first control is plasmid with insert, which resulted in full plate white...

07 August 2015 3,358 4 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

No title

02 March 2021 3,060 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View