I have been trying to figure out how to calculate what the concentration of a [H+] would be after you have exhausted the concentration of a buffer solution to figure out what the proper voltage would be in an experimental setup after it has reached equilibrium.
I do not know what the concentrations are as this is not explicitly stated in the paper. I have 0.02M of MES buffer with pKa ~6.15 on both sides of a liposomal flux experiment. Where the pH was adjusted using strong or weak acids to varying amounts
Say I move the outside and inside of the liposome to a pH of 4 on both sides, which is out of my buffer's range. How would I determine how many protons are in that pH 4 solution? The Henderson-hasselbach equation will fail since we would not have any conjugate base present at this point (and log(0) = undefined).
Is it just the concentration of buffer added (0.02) + 10^-4.0?
Thanks!
You should be able to calculate it using the Henderson-Hasselbalch equation, where pH is -log10[H+].
https://en.wikipedia.org/wiki/Henderson%E2%80%93Hasselbalch_equation
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