18 September 2022 0 7K Report

I'm looking at this study, https://www.biorxiv.org/content/10.1101/248179v3.full , and it uses a monoavidin - biotinylated donor DNA link to create a fusion of the donor DNA and the RNP so that they are colocalized at the cut site. With this they achieve like 90% HDR efficiency with lipofection, which is kind of crazy. Their best performing donor was their largest, and looking around I haven't found anyone else testing this system. Why isn't this more popular? Does this technique have a donor DNA size limit that I'm not aware of? I want to use it for a 6-7 kb knock-in.

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