Please tell me some good sites for MSA of proteins where I can get the data regarding structural parts identified with different colours. I know the basic ones like Clustal omega, T-Coffee etc, MUSCLE etc.
How can I make this type road network connectivity map in ArcGIS software? please help me Please see the map .
10 March 2024 8,483 2 View
I work in an enzymology lab focusing towards protein crystallography. We express our gene in pET-28(a) expression vector inside E.coli BL-21(DE3) host leading to protein expression. The problems...
20 December 2023 4,789 3 View
Please help me.
17 October 2023 4,437 4 View
I am having a trouble while trying to dissolve two compounds, one is Ebselen and another is Zofenopril calcium. I am trying primarily with DMSO. Ebselen is dissolving in 100uL DMSO but problem...
10 April 2023 5,979 1 View
Please give me socio economic geography research paper/journal link.
03 March 2023 9,623 2 View
Dear researchers, Do you have an idea how to precipitate FeOOH from a 2mM FeCl2.4H2O solution at pH 5.5 to 7? I know that adding NaOH in an FeCl2 solution would help precipitate FeOOH. However,...
24 January 2023 1,197 0 View
I performed a growth performance experiment of microalgae with four treatment. Where I measure cell dry weight (unit: mg/L), cell density (unit: ×10^5 cells/ml), chlorophyll a (unit: µg/ml) and...
13 September 2021 5,871 4 View
Dear Researchers, We are trying to impute values in a large data set (n=1950) by k-nearest method. There are 60 nos. of missing data which are needed to be imputed. The data will be used for PCA...
03 June 2021 5,013 3 View
Dear Researchers, We have found a hydrothermal association of biotite-sulfide (chalcopyrite-pyrrhotite-Co-rich sulfides)-uraninite-graphite-allanite with minor tourmaline and xenotime. We are...
07 May 2021 662 5 View
I have got 4-5 extra bands in SDS gel after running a my eluted protein which was purified through Ni-NTA column. This means impurities are present. My question is, can I again run my protein...
06 February 2020 2,819 2 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
Dear All, My lab primarily works on insect wing patterns. In one of the projects, my student and I have defined 19 abnormality characters on the forewing and 6 abnormality characters on the...
31 July 2024 6,464 5 View
I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...
28 July 2024 366 3 View
I have two groups that I test on three different tasks. I have 4 independent t-test, 6 paired t-test and 2 ANCOVA. My concern for which t-test should I conduct bonferroni correction. At the moment...
28 July 2024 7,827 6 View
Hey researchers! I am currently doing a research about to what extent in which A has accelerated the inclusiveness of the payment system in my country. Down below are a few examples of the...
26 July 2024 5,654 3 View
I am conducting a qualitative-driven approach to mixed-method research. The role of my quantitative data is to corroborate the findings of the qualitative data. Qualitative data has been collected...
24 July 2024 9,799 3 View
Hello! We are using LNP to deliver circular mRNA into T cells. However, compared with linear mRNA, the transfection efficiency and protein expression duration are similar. Are LNP generation or...
21 July 2024 4,322 1 View
Some time ago, I wanted to perform immunofluorescence on the S protein of mouse CD4 T cells using a Leica super-resolution microscope. I fixed with 4% paraformaldehyde, then added it to the well...
20 July 2024 3,797 2 View
It was assumed that 4E8 T cells should be isolated and activated using plate-bound anti-human CD3, after which would be used for a genome-wide CRISPR screen. (Ref: Genome-wide CRISPR Screens in...
17 July 2024 3,371 0 View
Coffee waste
16 July 2024 1,303 3 View