Subhecchha Baidya

3 Questions 4 Answers 0 Followers

Questions related from Subhecchha Baidya

What to do if I am getting a chunk type of filamentous growth of E.coli BL-21(DE3) cells in growth media?

I work in an enzymology lab focusing towards protein crystallography. We express our gene in pET-28(a) expression vector inside E.coli BL-21(DE3) host leading to protein expression. The problems...

21 December 2023 4,749 3 View

Does anyone know how to dissolve Ebselen and Zofenopril calcium?

I am having a trouble while trying to dissolve two compounds, one is Ebselen and another is Zofenopril calcium. I am trying primarily with DMSO. Ebselen is dissolving in 100uL DMSO but problem...

11 April 2023 5,949 1 View

Can I run a purified protein (with His tag) second time through Ni-NTA column?

I have got 4-5 extra bands in SDS gel after running a my eluted protein which was purified through Ni-NTA column. This means impurities are present. My question is, can I again run my protein...

07 February 2020 2,780 2 View