Subhecchha Baidya

6 Questions 4 Answers 0 Followers

Questions related from Subhecchha Baidya

What should I write to address this comment?

For MICs, it might be nice to also have the negative control included or express the MICs as fold-change relative to negative control. This is a comment from a reviewer of a reputed journal. My...

23 May 2025 9,048 1 View

What to do if I am getting a chunk type of filamentous growth of E.coli BL-21(DE3) cells in growth media?

I work in an enzymology lab focusing towards protein crystallography. We express our gene in pET-28(a) expression vector inside E.coli BL-21(DE3) host leading to protein expression. The problems...

21 December 2023 4,823 3 View

Does anyone know how to dissolve Ebselen and Zofenopril calcium?

I am having a trouble while trying to dissolve two compounds, one is Ebselen and another is Zofenopril calcium. I am trying primarily with DMSO. Ebselen is dissolving in 100uL DMSO but problem...

11 April 2023 6,010 1 View

Can I run a purified protein (with His tag) second time through Ni-NTA column?

I have got 4-5 extra bands in SDS gel after running a my eluted protein which was purified through Ni-NTA column. This means impurities are present. My question is, can I again run my protein...

07 February 2020 2,841 2 View

Sites for multiple sequence alignment (MSA)?

Please tell me some good sites for MSA of proteins where I can get the data regarding structural parts identified with different colours. I know the basic ones like Clustal omega, T-Coffee etc,...

28 January 2020 4,429 0 View

Is the Metallo Beta lactamase Bla2 (Bacillus anthracis 2) a mutant of Metallo Beta lactamase Bc-II (Bacillus cereus) ?

I am working on one of the metallo Beta Lactamases, named Bla2 (Bacillus anthracis 2) which is present in the BLDB (Beta Lactamase Database) in the same series of Bc-II. It is mentioned that Bla2...

03 January 2020 1,835 0 View