Centrality measures computation in Biblioshiny/ Vosviewer?

26 February 2021 7,279 2 View

Can we mix elemental powders of 325 mesh using an agate mortar and pestle?

Elemental Powders like Titanium, vanadium, tungsten, zirconium, hafnium.

04 January 2021 7,357 1 View

How to run spin polarized calculations in quantum espresso?

I would like to run spin polarized calculation using quantum espresso. Is it important to add spin polarization in calculating the wavefunction cutoff, K point convergence and geometrical...

17 November 2020 7,604 2 View

MAX phase synthesis?

We are having titanium, aluminium and graphite powders. But we don't have ball milling facility in our lab. Can we mix these manually using mortar and pestle for obtaining MAX phase powders? How...

16 November 2020 3,436 4 View

Material simulation of Concrete damage plasticity model(CDP), In abaqus?

09 November 2020 268 2 View

How to respond to the comments from multiple reviewers?

I have received comments from three reviewers. many of the comments are similar. do i have to respond to the comments of all the three reviewers individually or can i write a consolidated...

15 October 2020 9,220 4 View

Testing endogeneity in the model through VAR ?

I have the panel data regression model for which have to test the endogeneity. i read one research paper which was using Vector Autoregression (VAR) to test the endogeneity. in that paper, it...

03 October 2020 877 3 View

Enzyme assays of mutant GBA cell lines ?

How do i measure the enzyme activity of human GBA in cos 7 cells that have been transfected with mutant plamids. After 24 hours of plating cells in a 12 well plate, I transfect cos 7 cells with...

30 August 2020 4,496 3 View

How to describe mixed occupancies in the Quantum espresso input file?

I am having trouble describing partial occupancy in the input file. One of the site is occupied by two atoms with (50% occupancy). when I generate input file, the other atom is getting ignored....

19 April 2020 9,383 3 View

Antimicrobial properties of metal oxide nanoparticles?

08 April 2020 3,013 5 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

Cloudy/high background western blot - can anyone help me?

Hi everyone! I try to detect VDR protein (~48 kDa) using Western Blot. After SDS-PAGE using 10% MP TGX Stain-Free Gel from BioRad I transferred the protein onto an metOH activated PVDF membrane...

23 February 2021 6,266 3 View

How to improve the buffer capacity of sea water?

Hello everyone I would like to improve the buffer capacity of seawater (pH ~8) in order to dissolve as much CO2 as possible without dropping the pH below 7.5 (a range between 7 - 7.5 is...

17 February 2021 2,797 2 View

Why does flow deformation occur at the test line and control line on nitrocellulose membranes (LFIA)?

I use 1% BSA in 10 mM phosphate buffer (pH 7.4) solution when striping my nitrocellulose membranes with antigens and antibodies. However, I always observe deformed flow when my sample or running...

16 February 2021 4,224 3 View