Srikanth Konde

3 Questions 12 Answers 0 Followers

Questions related from Srikanth Konde

Is there any possibility to give negative results in ELISA? If my protein present in Urea solution

My protein present in 8M Urea, 1mM DTT, 1mM EDTA, 1mM PMSF, 10mM Nacl & 50mM Tris @pH 8.0 And it is buffer exchanged with 50mM Tris, 10mM Nacl, 1mM DTT,EDTA & PMSF @pH 8.0

30 January 2017 514 3 View

Is there any possibility of degradation of protein in 2M Urea?

My protein is recombinant one

19 December 2016 6,077 7 View

Sir/Madam please suggest me a proper protocol?

My protein is Cry1AC Mol.Wt 133kDa pI 5.0 (THEORITICAL) Protein Inclusion Bodies are forming. Please suggest me the buffers which can i use to 1. Cell Lysis (E.Coli, BL21DE3) 2. I.B Washes 3. I.B...

01 January 1970 9,349 3 View