Now a days most of the people having pet dogs in their home, Brucella canis is considered as a one of the potential zoonotic pathogen and it has greater public health significance.

As per my knowledge most of the serological assay kits utilizes the smooth phase Brucella species as their antigen substrates, but it won't detect rough phase B. canis antibodies. Even though culturing is considered as a gold standard test for Brucella canis detection, it is very laborious process we need wait upto 4 weeks for the results. There are some primers available to detect the B. canis but it is very expensive, for screening purpose we can't afford that much amount.

Is there any simple and economically feasible method to detect Brucella canis from clinical samples for screening purpose in dogs?

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