Ashraf E. Sayour

9 Questions 36 Answers 0 Followers

Questions related from Ashraf E. Sayour

Is there a simple method to separate immunoglobulin isotypes from blood serum?

I need to separate IgM, IgG and IgA from blood serum by a simple method/ kit.

03 April 2015 9,800 5 View

How can I verify the calibration of an ELISA reader?

I need to know the detailed procedure for verification of a calibrated ELISA reader.

18 March 2015 9,195 7 View

How can I verify the calibration of a centrifuge?

I need a detailed procedure for verification of a calibrated centrifuge.

18 March 2015 2,202 8 View

What is the best means for quantitative measurement of cytolysis?

I need to quantitatively measure cell lysis of bacteria that are already dead.

21 February 2015 2,560 1 View

Is anybody interested in collaboration studies with camel brucellosis?

All researchers are welcome to send camel sera from suspected areas together with retropharngeal and/ or supramammary lymph nodes. Sera will be screened with the buffered acidified plate antigen...

18 February 2015 1,473 3 View

Is there a recent alternative equipment to the classic Warburg respirometer?

Warburg respirometer is used for the determination of the oxidative metabolic pattern of Brucella on selected amino acids and carbohydrates by measuring the microliters of oxygen consumption per...

24 December 2014 935 1 View

Can I replace the technically demanding method of rat hepatocyte isolation by an easier alternative?

The standard method for isolation of primary normal rat hepatocytes is to cannulate and perfuse the hepatic portal vein first with Hank’s buffer for washing followed by collagenase in digestion...

29 November 2014 4,055 12 View

How can I choose the right combination of hemolytic units of complement fixation test reagents for titration?

The World Organization for Animal Health (OIE) recommends a minimum and maximum range of reagent concentrations to be followed by laboratories performing the complement fixation (CFT) for...

04 June 2014 5,255 2 View

How to separate serum from blood very quickly with minimal damage to the native complement?

It took me half an hour to separate serum from Guinea pig blood by allowing blood to stand still in a slant position at room temperature followed by centrifugation. I need a quicker means of serum...

04 June 2014 479 3 View