I would like to know If I want to see the biological effect of stromal (CAF) COL11A1 NOT cytoplasmic ,which one should I opt for silencing and overexpression assay or neutralizing COL11A1 antibody and exogenous recombinant human COL11A1?
I am trying to coat urea with Polyurethane modified with Siloxane and PEG-400. I synthesized the polyol myself using cotton stalks (Liquified cotton stalk) and the MDI that I am using is...
27 March 2024 5,587 2 View
I synthesized Mesoporous Nanosilica particles and then later loaded it with ZnO by impregnating it with Zinc acetate solution and then later decomposing it at high temperature, my XRD pattern is...
04 November 2023 1,478 3 View
04 November 2023 5,724 0 View
Hi, Dear Acarologists and everyone else. I hope this day will bring you joy, happiness, and success. I want to get your opinion. Which Software do you use to Illustrate mites or other arthropods?...
10 October 2023 433 3 View
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15 July 2023 8,552 1 View
I have got different patterns after I treat my biosensor with different inhibitors dose and identity. (Attached are three patterns). Which method should I use to identify that these dynamic...
13 September 2022 5,371 0 View
I synchronized the cells at G1 stage and then released them.with different cell cycle inhibitor. I found out that cells treated with G1 inhibitor has low no of cells in G1 phase comparing to other...
18 August 2021 6,820 0 View
Hi, I am working on biosensor with mvenous and mcherry. I fix the cells at different time points image them and then bleach it to remove the signals of sensor and then stain with Edu and DAPI....
03 June 2021 2,642 3 View
How do we design invivo experiment with isogenic cell lines. I want to see the effect of Carboplatin with and without COL11A1gene.? II am interested in OSE cells.
05 October 2020 543 0 View
Hi , I want to know that if I hypothesize that Gene A promoting chemoresistance in PDAC but what to do if I do not get any positive staining in my sample? What measure will I have to take which...
24 September 2020 7,465 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...
04 August 2024 4,255 1 View
We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...
31 July 2024 4,510 3 View
I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...
23 July 2024 6,664 6 View
I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...
22 July 2024 9,160 2 View
Hello, I recently stained my cells with an Arf6 antibody. The healthy control looks pretty normal, while the disease group cells have abnormally large black structures in the cell body. Has anyone...
21 July 2024 1,044 1 View
We have mice brains that were over-fixed due to old PFA used during perfusion. Thus, the synapses are no longer being labeled by the Synaptophysin (SY38) mAB. which works perfectly every other...
17 July 2024 7,767 3 View