I have got different patterns after I treat my biosensor with different inhibitors dose and identity. (Attached are three patterns).
Which method should I use to identify that these dynamic pattern are different or similar?
Hi , I want to know that if I hypothesize that Gene A promoting chemoresistance in PDAC but what to do if I do not get any positive staining in my sample? What measure will I have to take which...
24 September 2020 7,370 3 View
If I have only two options in front of me to select either Gasteiger or the AM1-BCC, so based on which parameters or rules I can select the most appropriate charge scheme during minimization step...
18 July 2020 8,763 1 View
Hi everyone, if I do not use spike-in RNA isoform controls for a customized RNA sequencing experiment (not the whole transcriptome, just based on few specific genes we are interesting), so is...
08 February 2020 7,190 2 View
Dear All, 1 ug of permissible quality FFPE RNA is transcribed to cDNA through SSIV VILO RT kit. I have Qubit v.4 device, which can measure cDNA to a good extent. The cDNA ranges from 25-100 ng /...
11 November 2019 2,009 1 View
i want to ask about the appropriate model for the prediction of 4 type of learning styles with the help of background variables of the respondents In my case the dependent as well as the predictor...
19 December 2018 7,237 3 View
It is a known fact now that interactions between bacterial (normal flora) and colon cancer cells influence the transcription profile of host cells or enterocytes. So, how can I avoid or minimize...
11 October 2018 9,483 1 View
If I am interested in a customized mRNA-only Sequencing (RNA-Seq) based on few transcripts (not the whole transcriptome), and after having satisfied & permissible RIN values for my extracted...
21 September 2018 1,804 3 View
Dear all, in a PCR (conventional & realtime both), if we achieve our desired product (GOI) and along with, there is also no primer dimer (PD) formation seen on the gel and melt curve, does...
02 November 2017 9,949 3 View
Any explanative thoughts or information? Will the 2nd or 3rd melt of products attribute to some improvement for their detection?
20 October 2017 8,562 3 View
Greetings! I'm working on two strains (PAO1 and PAK) of the same bacteria, Pseudomonas aeruginosa. I have obtained the growth curves of the two strains separately to have the OD vs CFU/ml curves...
02 May 2017 8,123 2 View
My study consists simple corrosion inhibitors in salty brine. Right now I just fit my circuit with randles with CPE. Just wondering how the slop can given more info of the experiment. Or any...
01 March 2021 6,474 3 View
Hello everybody, I am investigating the influence of a toxinon various clinical-chemical and haematological parameters in fish. I have three treatments (control, low dose, high dose) in my...
24 February 2021 1,243 1 View
Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...
23 February 2021 7,429 1 View
I am planning to perform a degranulation assay using CD107a antibody. Some resources suggest to use inhibitors like monensin to inhibit the intracellular traficking and acidification of the...
21 February 2021 6,260 3 View
We want to use phenol pH graphic to measure the pH of mediums before and after inhibitor application to cells. We have phenol absorbence values at 460, 560 and 720 nm wavelength. When we measure...
20 February 2021 7,700 3 View
I'm planning to use ST078925 OGT inhibitor from TimTec (OGT-O-linked N-acetylglucosamine (GlcNAc) transferase) for female rats.
16 February 2021 7,850 2 View
Hi, I'm asking from the point of view of designing a research plan: How long (approximately) can it take to determine the transition state structure of an enzyme substrate using kinetic isotope...
14 February 2021 2,610 1 View
I came across with this study here, https://www.clinexprheumatol.org/abstract.asp?a=5066 And this study says: "The sample size calculation was done to compare two different MTX starting dose...
14 February 2021 3,017 3 View
Currently I'm doing a literature review regarding the comparisons of different types of radiation such as uv, electron beam and gamma radiation. But I'm having a difficult time converting uv unit...
11 February 2021 7,642 3 View
We aim to use MK-2206 to inhibit AKT in mice. However, a large body of studies suggests that it should be administered orally. I was wondering if somebody could tell me that is it toxic for the...
07 February 2021 6,340 1 View