How do we design invivo experiment with isogenic cell lines. I want to see the effect of Carboplatin with and without COL11A1gene.? II am interested in OSE cells.
I am trying to coat urea with Polyurethane modified with Siloxane and PEG-400. I synthesized the polyol myself using cotton stalks (Liquified cotton stalk) and the MDI that I am using is...
27 March 2024 5,587 2 View
I synthesized Mesoporous Nanosilica particles and then later loaded it with ZnO by impregnating it with Zinc acetate solution and then later decomposing it at high temperature, my XRD pattern is...
04 November 2023 1,478 3 View
04 November 2023 5,724 0 View
Hi, Dear Acarologists and everyone else. I hope this day will bring you joy, happiness, and success. I want to get your opinion. Which Software do you use to Illustrate mites or other arthropods?...
10 October 2023 433 3 View
??
15 July 2023 8,552 1 View
I have got different patterns after I treat my biosensor with different inhibitors dose and identity. (Attached are three patterns). Which method should I use to identify that these dynamic...
13 September 2022 5,371 0 View
I synchronized the cells at G1 stage and then released them.with different cell cycle inhibitor. I found out that cells treated with G1 inhibitor has low no of cells in G1 phase comparing to other...
18 August 2021 6,820 0 View
Hi, I am working on biosensor with mvenous and mcherry. I fix the cells at different time points image them and then bleach it to remove the signals of sensor and then stain with Edu and DAPI....
03 June 2021 2,642 3 View
Hi , I want to know that if I hypothesize that Gene A promoting chemoresistance in PDAC but what to do if I do not get any positive staining in my sample? What measure will I have to take which...
24 September 2020 7,465 3 View
Hi, I am designing my proposal on gene which cause chemoresistance in cancer. From literature review I have come up with a gene COL11A1 which has previously been identified as a chemoresistance...
24 September 2020 9,063 1 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
I'm working on carboplatin which i bought from local pharmacy. It is a Carboplatin injection IP 150mg/15ml which is sterile aqueous solution for intravenous use. Unfortunately, I cannot able to...
04 August 2024 454 3 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...
03 August 2024 3,851 1 View