I am looking for a good antibody to use as loading control for rabbit's proteins in Western blot. Any recommendations?

I tested two primary antibodies anti-beta-actin so far, and I am getting very inconsistent results accross my Western blots, sometimes I don't even get any detection, and sometimes I get...

02 December 2019 493 2 View

Does anyone working with Renin (Renin-Angiotensin system) know the normal values of renin in plasma of rats?

I am working with female rats and want to run an ELISA for renin, however I need to know the level of renin in plasma of healthy rats to choose a kit with a suitable sensitivity. Thanks!

06 March 2019 175 3 View

Any recommendations on ELISA kit to measure Renin in plasma samples of rats?

Hello there! I am looking for an ELISA kit to measure renin in plasma of rats (later I'll analyze rabbit samples, will need a good kit for that as well), control versus dehydrated are my...

04 March 2019 2,972 2 View

Do we need to wash our cells treated with drug before using DHE?

I have to treat my cells with DHE do i need to wash the cells after treating with the drug and then i have to treat cells with DHE?

11 July 2016 7,001 4 View

Can I insert two genes (from different organisms) which encode the same type of protein, in a single vector ?

10 June 2016 3,664 3 View

What is the difference between char yield and residual mass?

17 February 2015 7,676 4 View

How we can decide the dose for different animal models?

how we can calculate and decide the dose for different animal models?

01 January 1970 2,034 3 View

Any information about interactive media for learning traditional dance?

Museums in town tell about history and culture. We need some interesting media to attract them, giving experience beside learning culture and history. especially for children. Any Reference or...

01 January 1970 9,040 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View

LOAD DISPLACEMENT CURVES Stiffness increasing in ANSYS non linear analysis. HOW CAN I CORRECT IT?

Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...

01 March 2021 9,911 3 View

How to choose ANN number of hidden layers and nodes?

Hello, Could you please share any interesting research explaining how to choose the number of hidden layers and nodes per layer in case of regression problems using ANN? Thank you, any help would...

01 March 2021 6,200 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

How could I take into consideration the weights of observation in a system of seemingly unrelated regressions (SUR) in rstudio?

I build a model of 4 regression equations (probit and OLS) in R studio. I need to take the weights of observations into consideration. While it is simple to do so for every regression equation,...

27 February 2021 1,233 1 View

Can random sampling have inclusion criteria?

As random sampling means that everyone in the population have an equal chance to be chosen, can it have inclusion criteria? My research participants have been chosen based on the inclusion...

26 February 2021 6,905 10 View

Do you kow of any database containing data on retention time, log P and polarizability for a large number of molecules?

Hi, I am a chemistry undergraduate doing my dissertation project on HPLC. In my project, I need to predict the HPLC retention time of 20 aromatic molecules, the calibration set. To do this I will...

24 February 2021 2,602 1 View

How to run raise regression in R?

if anybody know how to run raise regression in r, and raise parameter selection, please help me

24 February 2021 8,199 1 View

Can anyone suggest me the best way for Regression analysis of Accidents?

Dear Altruist, I want to make a regression analysis based on accident data. But I am not finding the necessary information about what type of regression is more applicable for analysis accident...

23 February 2021 9,956 3 View