The Joule-Thomson effect?

Hello I recently am suffering calculation of temperature change resulted from the Joule-Thomson effect when propylene gas at 35 oC at 2 bar passes through a membrane to vacuum pressure (~ 0 bar)...

23 December 2020 4,091 2 View

When available, are you planning to receive SARS-CoV-2/COVID-19 immunization? What do you most fear about it?

If possible, let us know about the which vaccine would be available in your geographic location.

06 December 2020 3,527 23 View

How to invite call centers to participate in your study?

I'm conducting a job satisfaction study to predict telephone agents' turnover. I'm looking to increase participants for my study. How should I approach the directors of call centers to request...

20 September 2020 7,099 8 View

What is the correct statistical test for this data?

I have data on a bacteria (Group B Strep) that colonizes women but it can also cause infection (it's a sneaky little bug). I want to see if there is a difference between pregnant and non-pregnant...

13 August 2020 5,805 10 View

How to avoid incomplete responses?

I'm conducting an anonymous quantitative survey on SurveyMonkey and some participants left the majority of the questions unanswered. How can we encourage participants to completely answer all...

22 June 2020 8,202 4 View

How to calculate specific capacity in C/g from a CV curve?

I am newbie to battery materials. As I understand, specific capacity of a battery-type material can be expressed in term of C/g or mAh/g and can be calculated from the cyclic voltammetry (CV) or...

06 May 2020 4,202 6 View

Have you applied 3D Laser scanning and Building Information Modeling for facility management during operation phase?

14 March 2020 7,806 3 View

I am looking for a good antibody to use as loading control for rabbit's proteins in Western blot. Any recommendations?

I tested two primary antibodies anti-beta-actin so far, and I am getting very inconsistent results accross my Western blots, sometimes I don't even get any detection, and sometimes I get...

02 December 2019 493 2 View

EDS Mapping how to get better results?

Hi everyone, hope you're all okay. So I have some questions/doubts about eds mapping. Just some notes first: I always use the correct accelerating voltage to excite the elements I need, always use...

04 June 2019 2,854 11 View

Histogram on a Scanning Electron Microscope, how to interpret?

Hey folks, hope you're all okay. I have one doubt, I always used to check the videoscope to help improve the bright and contrast in the microscope, but I used to do this in a FEI (Thermofisher)...

17 May 2019 4,055 4 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

Has anyone tried to scan fluorescence IHC slides with microarray scanner?

I have an old slide microarray scanner (VIDAR Revolution 4200) and would like to give it a "second life", as IHC scanner array (fluorescence dyes), FISH over tissue or similar. Has anyone tried...

24 February 2021 9,210 1 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

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22 February 2021 9,932 11 View