12 October 2021 1 2K Report

As the title suggests, should SDS and/or methanol be included in the transfer buffer for transferring large molecular weight proteins (>300 kDa)? If so, how much of each is optimal?

For context, I am using 3-8% Tris-Acetate gels and transferring to 0.45uM nitrocellulose membranes overnight at 4degC.

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