Should I use triple distilled water for HPLC-DAD analysis?

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How can, granulometry or grain size data, be used in enrichment factor calculations?

As a stable element

23 July 2024 4,187 1 View

Is there anything faster than Xarray or Pandas out there?

Hello, dear RG community. Personally, I have found Xarray to be excruciatingly slow, especially for big datasets and nonstandard operations (like a custom filtering function). The only suggestion...

15 July 2024 4,705 2 View

O impacto da Gestão Estratégica de Marketing, na tomada de decisões empresariais?

o impacto da Gestão Estratégica de Marketing, na tomada de decisões empresariais?

10 July 2024 3,430 0 View

My hippocampal mouse primary neurons clump when I plate them on microfluidic devices for axonal isolation. Any tip?

Hi all, I am working on both primary hippocampal mouse neurons and i3Neurons to isolate axonal material. I plate them in microfluidic devices in house made (like any other you can find on the...

01 July 2024 8,070 0 View

Can you use MTT assay for tissue samples?

Can you use the MTT assay of cell viability for tissue samples derived from rats e.g. liver, muscle, heart, brain, or is this method reserved for cultured cells? Thank you

29 May 2024 6,389 2 View

What are the features and best library to predict and clasify people?

Hello everyone, I am currently exploring several options to give the collected data the greatest value possible. I have demographic data on older people, where I perform various memory and mood...

02 May 2024 876 6 View

Is this formula to calculate LST is accurate?

Could you please provide me some authoritetic refers where this formula used if it accurate OR tell me why it is wrong and how to fix it? 1. TOA (L) = ML * Qcal + AL 2. BT = (K2 / (ln (K1 / L) +...

19 April 2024 8,924 0 View

How to derive the volume based equation for gas fraction starting from Dr. Ishii's definition of local gas fraction?

Hello, dear RG community. Very often, gas fraction (also known as void fraction or gas holdup) is thought of as a ratio of the gas volume to the total volume:...

04 April 2024 7,290 0 View

Spheroid or not to spheroid? That is the question?

Dear Researchers, I am reaching out to this esteemed community seeking insights into a recent experiment I conducted involving cell seeding in agarose micro-wells. My aim is to engage with fellow...

22 March 2024 4,438 0 View

Do you know of a specific videogame or genre of videogames that stimulates metacognition?

I'm researching about videogames and their effect on metacognition, actually I intend to develop a game with that specific intention. I'm tinkering with a puzzle game that gives feedback and maybe...

18 March 2024 1,147 2 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

How is surfactant addition calculated?

I want to make 100 ml of MWCNT/distilled water mixture at a volume fraction of % 0.1. MWCNT density is 2100 kg/m3. I will add gum arabic as surfactant. If I add surfactant at a 1:1 ratio, will the...

19 July 2024 8,494 0 View

How can we separate diastereomers of larger organic moiety?

For example, oxazine type moieties which forms diastereomers, how the separation can be done? Chiral HPLC is the only possibility?

10 July 2024 1,519 3 View

How i can calculate mathematically COD concentration if i add 1gr from glucose to 1 L distilled water ?

how i can calculate mathematically COD concentration if i add 1gr from glucose to 1 L distilled water

08 July 2024 9,104 2 View

Is there any guidelines about zooming out a chromatogram?

Hi, Just curious to know that in chromatography, a chromatogram should be zoomed to what scale? 10 times of output , 50X or ..? Is there any regulation or general chapter which describes this?...

03 July 2024 3,119 6 View

Can u pls send me the conversion n selectivity formula for HPLC analysis?

please answer it quickly.

24 June 2024 2,256 2 View

What C18 HPLC mobile phase can separate L-leucine oligomers?

After condensation of amino acids (they do not contain any protection groups), I believe that I have produced leucine peptides such as di-leucine, tri-leucine, leucine diketopiperazine,...

16 June 2024 2,091 1 View

How can I remove potassium carbonate from amino acid condensation reaction?

My amino acid condensation reaction (the reagents do not have protection groups) utilizes K2CO3 as a base. K2CO3, the resulting peptide product and unreacted amino acids all have decent solubility...

16 June 2024 7,723 3 View

What should we do to solve the clogging problem of Superose 6 Increase 10/300 Column?

We have a Superose 6 Increase 10/300 column (recent photo attached). Recently, we noticed that when we increased to our normal flow rate of 0.5 ml/min, the column reached the pressure limit....

04 June 2024 8,226 0 View

Will there be colour generation in the control of Cysteine-Carbazole reaction?

The control contains the buffer, distilled water and the sugar only.

29 May 2024 6,941 0 View