I have the sf9 cell culture in Sf-900 II SFM medium supplemented just with 0,1% Gentamycin. The cells are not growing well and I read some advices that the FBS shall be added. Should I use the penicillin and streptomycin instead of gentamycin?
Hi Ling,
Please have a look at this RG thread, this issue has been discussed already and you should find some useful info.
Hope this helps. Good luck. Norbert
Hello Ling: Use penicillin and streptomycin, the cell culture will be better,
Hi Ling Ii,
my personal view is to not use any antibiotics. They only help to mask an infection that can build up resistance until it explodes. With good aseptic technique you shouldn't get any infections, and if you do they are easy to spot. (This is a debate as old as cell culture :) )
You don't need any FBS when using SF900II medium. The only reason to add FBS is for virus storage, and this can be added post production.
What are your growth conditions for your Sf9's? Perhaps there's some other reason for their poor growth.
-Richard
Hi Ling,
I don't use antibiotics. I grow the cells in the same medium in these conditions:
*shaker flasks at 100-120 rpm;
*27°C;
*no CO2;
*density range: 5x105-3x106 cells/ml.
Alessandro
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