I prepared medium with DMEM powder (Cat#D5468, Sigma) and add NaHCO3 (3.7g/L). Since the pH will fluctuate due to CO2, should I adjust the pH of DMEM when I prepare it or not?
Thank you for answering this! I am at the elementary level of cell culturing. Hope that answering this won't bother you. I am wondering since the CO2 will volatile into the air during titration , how can I make sure that pH will come to the target value when I incubate medium under the 10% CO2. Will it become more acidic then I predict? if so, how to culculate the amount of HCl to be added ?