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Questions related from Pin-Chun Chen
I prepared medium with DMEM powder (Cat#D5468, Sigma) and add NaHCO3 (3.7g/L). Since the pH will fluctuate due to CO2, should I adjust the pH of DMEM when I prepare it or not?
18 July 2021 3,949 3 View
1. I always get no signal at partial region of promoter of pCAGEN after sequencing. I have tried couples of primer before (forward))/after (reverse) that region but it still can't work.
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