Hello,
Since one year I have been doing some experiment and storing pull downs at -80, eluting samples with elution buffer. I am using these elutes for kinase assay then, and do not get enough luminescence, mostly getting negative values for the raw data and cannot it even.
Is it possible because I am storing sample protein in elution buffer at -80 that's why this is happening. Do you recommend storing eluted protein at -80 or -20?
Storing them at -80 is preferable to -20 for long-term retention of activity. When freezing them, you should flash-freeze, which means freezing them quickly so that the solutes do not separate from the ice. You can do this with liquid nitrogen, or a dry ice-ethanol bath or, my preference, placing the samples in dry ice powder.
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