Arooje Nasir

6 Questions 3 Answers 0 Followers

Questions related from Arooje Nasir

Shall we store Pulldown elute at -20 or -80?

Hello, Since one year I have been doing some experiment and storing pull downs at -80, eluting samples with elution buffer. I am using these elutes for kinase assay then, and do not get enough...

16 February 2023 3,061 1 View

Does someone know good easy-to-work protocol for Death assay?

Hello, I am working with M93 cells, and need to run death assay with them, looking for a good easy-to-work protocol.

06 October 2022 4,836 0 View

Can someone guide me how to plate cells for performing a death assay?

Hello, I am planning to perform a death assay with M93 cells and looking for a good protocol, and in addition, how to plate cells for a death assay?

06 October 2022 2,882 1 View

Do we need to have drug compounds at room temperature, if we are using them with Promega Kinase assay kit?

Hello, I am using Promega ADPglo Kinase assay kit, there is alot of variability in my expeirment results, sometimes inhibitors work and show inhibition of kinase activity, and sometimes they...

06 October 2022 6,657 1 View

How to do the calculations for diluting a protein sample to make it of the same concentration to other protein for pull down?

Hello, I am doing Pull down assay, and I have two proteins. Protein1 concentration is 952.62ng/ul and Protein2 concentration is 1,015.092, I want to take input of 200ul (190ug) for both of them...

22 April 2022 6,940 3 View

BCA Assay for calculating protein concentration?

Hello, can someone help me or guide me through BCA assay, what formula is used in the end to calculate the protein concentration.

29 January 2022 6,625 4 View