I am trying to set up our lab for human DNA extraction from brain tissue and CSF. Any suggestions on how to go about setting up a clean room to prevent any external contamination from bacteria, fungi and viruses? Thank you!
- Large ionizng hepa filter blower
- 8x4 foil-coated styrofoam sheets
- Caulk gun & aluminum duct tape to seal the cracks
- 8w undercabinet lights with the corresponding UV sterilizing-grade light bulbs (turn UV on only if neither you nor your desired DNA samples are in the room)
All to be had for under $500 at home depot.
It would be good to know what you're starting with. Besides the above suggestions, which are okay but will not a clean room make, positive air pressure and a garbing room would help. Good training in aseptic technique for personnel is a must. And not "regular old" training - actual training (to industry, not academic standards).
i really like the settings of this video and i wanna share it with you if it will help, thanks
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