Hello
May I know the cost for the Sequencing Whole Genome for DNA bacteria.
Adding to Brian Thomas Foley 's answer, whole genome sequencing now a days is nothing in terms of time and efforts/understanding required to assemble and annotate the genome. Though there are several pipelines which can do everything automatically for you, but still, there need to be expertise required to assemble the contigs.
Cost is dependent what platform you are using for sequencing. That also indicate different types of data and data analysis/computing power. This setup will cost the least. Furthermore, there are companies which will provide an A to Z solution, thus you can imagine the addition of the cost modules for each extra service (for example https://www.abmgood.com/Whole-Genome-Sequencing-Service.html). Thus, i dont think you can get any specific answer for this question. May be you can enlist your preference, than you might reach any cost figures.
Sorry, I do not know the answer, but I hope the people who give you answers indicate if the cost includes a good assembly and any annotation or not. I am pretty sure today that assembly and annotation can take more time and effort than the sequencing, and that a good assembly requires a high quality sequence.
But for many purposes, assembly and annotation are not needed at all, so you may be able to use raw short reads if you are not interested in a completed genome.
Adding to Brian Thomas Foley 's answer, whole genome sequencing now a days is nothing in terms of time and efforts/understanding required to assemble and annotate the genome. Though there are several pipelines which can do everything automatically for you, but still, there need to be expertise required to assemble the contigs.
Cost is dependent what platform you are using for sequencing. That also indicate different types of data and data analysis/computing power. This setup will cost the least. Furthermore, there are companies which will provide an A to Z solution, thus you can imagine the addition of the cost modules for each extra service (for example https://www.abmgood.com/Whole-Genome-Sequencing-Service.html). Thus, i dont think you can get any specific answer for this question. May be you can enlist your preference, than you might reach any cost figures.
Hi Shaima, I agree with Brian and Abhijeet, pure sequencing is the easiet (and cheapest) part; everything else depends on what you need it for.
If you want to sequence just a different strain of an already sequenced species, then it's much simpler since you have a template to guide your assembly.
If, viceversa, your bacteria is brand new (not sequenced by anyone before), then you need de novo assembly and related gene annotation, which is not very difficult (there is software to do that, or alternatively you can ask the sequencign company to do it for you -with increasing cost) if it is a pure isolate. If it is, let's say, mixed sample (like environmental isolate), then assembly can be much more complicated. There are different sequencing methods, usually 3 are the most used: Illumina (I would suggest MiSeq that could overcome some complex regions, if it is de novo), IonTorrent or PacBio, with the last being the most expensive and maybe excessive for the task. However if you perform some literature search you'll find the best approach to use for your needs
Hi,
I agree with all colleagues, also it depends on your project
You can visit macrogen website, resume your project then a manager will contact you
https://dna.macrogen.com/eng/support/ngs/intro/wgs.jsp
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