Marco Pietrella

5 Questions 12 Answers 0 Followers

Questions related from Marco Pietrella

Working DNA extraction from ethanol-stored leaf samples?

Hi, I have to perform genomic DNA extraction from mulberry leaf samples that have been stored for a couple of years in ethanol (unknown concentration) presumably at RT so that part of the samples...

11 August 2020 4,498 5 View

How to identify mutations between two NGSequenced samples?

Hi, we have two Illumina-sequenced plant samples where one is a mutant of the other. Do you know a straightforward method to compare these in order to spot small (SNPs, InDels) or maybe longer...

21 November 2018 6,010 4 View

How to assess sample identity from 2 different RNAseq dataset?

Hi I got RNAseq data from what I suppose to be the same accession of a plant species (no genome available) sampled in different geographical regions. Do you know a reliable method to assess...

05 October 2017 6,051 2 View

Suggestions of free software for genetic linkage analysis?

Hi all,I need to perform linkage analysis (basically what JoinMap can do) and to draw related maps for plant (fruit-trees) species and I ask you for reliable freeware that could do that job. Thanks!

23 March 2015 9,250 4 View

How to correlate fruit developmental time series between closely related organisms (varieties) to find differentially expressed transcripts?

I am looking for a way that allows me to pinpoint genes that are significantly up- or down-regulated among such samples during the development (maturation) of a fruit. I thought of DESeq, but it...

05 May 2013 6,034 3 View