I am interested in probing flux thru different metabolic pathways (pentose phosphate, glycolysis, OxPhos, amino acid metabolism, cholesterol biosynthesis) in a host cell in response to intracellular infection. I am not sure if there is a way to separate what's going on in the host cell from what's going on in the microbe--but I'm interested solely on what is going on in the host cell. Anyone have any experience separating the two? Many of the common assays, like glucose uptake, extracellular acidification, would not be able to differentiate host from intracellular microbe metabolism. Thanks in advance