Hi all,
I am currently in need of a way to propagate Sendai virus (Cantell strain ATCC VR-907). I am aware that the traditional method of propagating Sendai is by infecting embryonate chicken eggs and harvesting the allantoic fluid, but I would prefer to avoid this method if possible. I am curious if anyone has had success in propagating Sendai in cell culture.
I have tried to propagate in the following conditions with no success:
MDCK CRL-34 with 3ug/mL Trypsin/TPCK
MDCK.2 with 3ug/mL Trypsin/TPCK
Vero with 3ug/mL Trypsin/TPCK
Regardless of the time of incubation (out to 7 days) I have failed to detect CPE to any notable amount and the supernatants also fail to hemmaglutinate chicken RBCs (the stock virus has a very high HA titer).
I have seen some information on the addition of DEAE-dextran to improve infection but I have not tried this.
Does anyone know of any protocols or have any experience with propagating Sendai virus in cell culture?
you can find some information in the link below:
Article Efficient propagation of single gene deleted recombinant Sen...
Hi Alex,
You can use primary pediatric bronchial epithelial cells for cultivation of Sendai virus. You can get more information in the paper below:
http://jvi.asm.org/content/84/22/11718.full
Caco-2 cells are capable to generate high yield of Sendai Virus.
Article TMPRSS2 Is an Activating Protease for Respiratory Parainflue...
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