Alex Jureka

7 Questions 3 Answers 0 Followers

Questions related from Alex Jureka

DE3 Bacterial cell lysis conditions in Branson SFX150 Sonicator?

Hi All, We are interested in using the Branson SFX150 sonicator we have in our lab to lyse DE3 E.coli in order to purify a couple of proteins. I have worked with larger sonicators in the past to...

29 May 2019 3,046 2 View

Will heat treating cell culture supernatants effect their performance in downstream applications such as ELISAs?

Hi everyone, I am currently trying to work out inactivation procedures for influenza virus. I am interested in using heat treatment for cell culture supernatants, but I'm concerned that heating a...

19 June 2018 4,777 3 View

Sendai virus propagation in cell culture possible?

Hi all, I am currently in need of a way to propagate Sendai virus (Cantell strain ATCC VR-907). I am aware that the traditional method of propagating Sendai is by infecting embryonate chicken...

01 March 2018 7,454 4 View

Ok to freeze cell lysates if looking for protein-protein interaction by 6xHis pulldown?

I am currently working on optimizing a protocol for determining if two proteins are interacting in mammalian cells using pull downs. The protein of interest being over expressed in the system will...

23 January 2018 3,167 3 View

Is it possible to use native gel electrophoresis for two proteins with varying pI's to determine interaction?

We are currently trying to identify a protein-protein interaction utilizing Native gel electrophoresis with purified proteins. The two proteins have quite different pI's, one around 5.5 (12 kDa)...

22 June 2015 9,299 11 View

Program for aligning protein sequences to reference sequence?

Hi everyone, I am currently trying to align several thousand (5-10) protein sequences to a related protein sequence (reference) to determine the percent identity of the large subset of sequences...

21 August 2014 5,813 10 View

Is it possible to do intra-cellular staining for only proteins in the cytoplasm (no permeabilization of the nuclear membrane) for flow cytometry?

I am currently interested in doing some flow cytometry to accompany confocal protein co-localization studies in order to get a more quantitative measure of the co-localization. The problem is, we...

07 May 2014 7,055 3 View