i have two oral cancer cell lines and 3 different drug (cisplatin, docetaxol and 5fu)single resistant sublines of both the cell lines.
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Now i am confused how to select the right endogenous control. Should it be Common for all as in row1, or drug based or cell line based?? As I see difference in gene expression based on endogenous control selection.
Jacques Gilloteaux
Why the genes, always the genes...How about DNase low activity?
See HS Taper references long ago also and Daoust R> Tumor cells that are resistant have lowest DNase activity as well as RNase activity. Papers are accessible through internet at pubmed and J Histochemistry and Cytochemistry>
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