Iris Steitz

3 Questions 2 Answers 0 Followers

Questions related from Iris Steitz

Does lysozyme also affect nucleases like RNase?

I'm wondering if lysozyme could also affect nucleases like RNase. My understanding is that lysozyme hydrolyses glycosidic bonds in bacterial cell walls, specifically between N-acetylmuramic acid...

07 December 2023 2,772 2 View

Seeking Expert Insight: Can anyone help us find a citation that confirms the functionality of RNase A under varying salt conditions?

Many enzyme suppliers claim that RNase A cleaves both single and double-stranded RNA under low salt conditions. However, at higher salt concentrations, it's said to cleave only single-stranded...

16 October 2023 7,464 3 View

What is the best alternative to Q5 polymerase for long-range PCR and what protocol do you use?

I'm trying to do a long-range PCR (~10kb) and I'm just wondering what polymerase to use despite the Q5. Do you have any experience in doing a long-range PCR with other polymerases? Did you use the...

31 May 2023 9,726 3 View