Recently we’ve noticed these patterns on our separating gel 15% and I’m wondering if anyone can provide insight into it. Is it normal? And if it isn’t what could we possibly be doing wrong?
We’ve already changed our Temed, made a fresh new AP and buffer too.
Hello
It might be occurred if any delay occure between addition of TEMED and pouring the gel into cast. It cause gel polymerization.
Moreover, you ensure that all SDS-page buffers have room temp. Before mixing them together.
It is better that APS and SDS prepare refresh.
It looks to me as if the clips holding that corner of the glass plates together are releasing during the run and the plates are moving apart in that corner and the gel has become detached from the plates and is sticking partially to both plates. Try tightening the clips and washing the gel spacers in case they are dirty.
”hi, do you pour your gel from one point? This also looks like you choose one point and pour gel to the cast. i would recommend you to move through the cassette when you are pouring gel. If you continuously pour gel from one point, the first amount of gel starts polymerization and if you continue to add more gel under this part, the weight causes shrinkage . So you can try to move your pipette during gel pouring from one side to other side of the cassette. And be sure that you are pouring your gel immediately before your mix is ready.
Dear Robert Adolf Brinzer Fatemeh Yaghoobiadeh Paul Rutland Nazlıcan Yürekli thank you all so much for your valuable insights. I’ll for put it in practice for the next time and hopefully it will work ☺️
- Badges
- Science topic
- Similar topics
- Philosophy
- Philosophy Of Science
- Reasoning