ScRNA seq data derived from PBMCs of SSc patients

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Tumor necrotic zones - How do they look like in Hematoxylin - Eosin staining?

When Hematoxylin - Eosin staining is performed in mice tumors, which are the most important tumor tissue characteristics that determine the existence of necrotic zones? What changes in tumor...

24 April 2024 3,656 1 View

Tumor monitoring - Difference between H&E staining and BLI?

I am comparing the results of ex vivo BLI and H&E staining for mouse organs. Some organs appear to have tumors in BLI results but not in H&E or the opposite. Why this can happen?

06 February 2024 9,832 0 View

Interested in Exploring a Metamathematical Approach to the SIR Model in the Context of the Hong Kong Flu in NYC?

Dear ResearchGate Community, I am currently working on a research paper titled "A Metamathematical Approach to the Susceptible-Infected-Recovered (SIR) Model: The Case of the Hong Kong Flu in New...

05 August 2023 9,037 4 View

How much T-bet do B cells express in healthy humans??

I have some flow cytometry issues with T-bet staining, thus I would like to ask about the transcription factor's approximate percentage (a mean, actually ) in peripheral blood samples from healthy...

08 October 2022 2,416 2 View

How to remove EDTA from stored plasma cells?

We are performing FISH in plasma cells. When it comes to back up, we are using a small amount of EDTA in order to store those cells. In case of a repeat, the EDTA creates a kind of membrane in the...

29 June 2021 8,989 1 View

Need for ivc filter?

I would like expert opinion on the following case: 84 yo male, with intermittent episodes of painless jaundice under investigation and known Hx of polycythemia rubra vera, under effective...

16 January 2021 1,449 6 View

What are some good tDCS machines for M1 and cerebellar stimulation?

In the context of visuspatial skill/sequential motor learning if that is of any help or relevance. Thank you in advance for any responses.

18 February 2020 1,887 0 View

Is there any human B cell line which serves as a model of autoimmunity??

I know it's not easy to find such a cell line (most of them are models of cancer afterall and it's also impossible for a cell line to produce autoantibodies), but I want to perform some in vitro...

10 January 2020 8,069 0 View

Has anyone successfully used the EasySep Human CD19 Positive Selection Kit II from STEMCELL Technologies??

I use human blood samples (~8ml each) from healthy individuals and perform centrifuge over lymphoprep, in order to isolate the PBMCs. Then, I use the EasySep Kit to isolate the CD19+ cells. The...

17 November 2019 8,890 3 View

Has anyone successfully stained (intracellular staining) for T-bet with the Cytofix/Cytoperm Kit from BD Biosciences??

I isolate PBMCs from human blood and try to perform intracellular staining for T-bet (i'm interested in CD19+ cells, but for now i use CD3+ as a control). I do know there are kits that are...

22 September 2019 3,210 3 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Why do we equate male and female arousal?

Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)

05 August 2024 9,537 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View