I am comparing the results of ex vivo BLI and H&E staining for mouse organs. Some organs appear to have tumors in BLI results but not in H&E or the opposite. Why this can happen?
When Hematoxylin - Eosin staining is performed in mice tumors, which are the most important tumor tissue characteristics that determine the existence of necrotic zones? What changes in tumor...
24 April 2024 3,656 1 View
Dear ResearchGate Community, I am currently working on a research paper titled "A Metamathematical Approach to the Susceptible-Infected-Recovered (SIR) Model: The Case of the Hong Kong Flu in New...
05 August 2023 9,037 4 View
I have some flow cytometry issues with T-bet staining, thus I would like to ask about the transcription factor's approximate percentage (a mean, actually ) in peripheral blood samples from healthy...
08 October 2022 2,416 2 View
We are performing FISH in plasma cells. When it comes to back up, we are using a small amount of EDTA in order to store those cells. In case of a repeat, the EDTA creates a kind of membrane in the...
29 June 2021 8,989 1 View
I would like expert opinion on the following case: 84 yo male, with intermittent episodes of painless jaundice under investigation and known Hx of polycythemia rubra vera, under effective...
16 January 2021 1,449 6 View
In the context of visuspatial skill/sequential motor learning if that is of any help or relevance. Thank you in advance for any responses.
18 February 2020 1,887 0 View
I know it's not easy to find such a cell line (most of them are models of cancer afterall and it's also impossible for a cell line to produce autoantibodies), but I want to perform some in vitro...
10 January 2020 8,069 0 View
I use human blood samples (~8ml each) from healthy individuals and perform centrifuge over lymphoprep, in order to isolate the PBMCs. Then, I use the EasySep Kit to isolate the CD19+ cells. The...
17 November 2019 8,890 3 View
I isolate PBMCs from human blood and try to perform intracellular staining for T-bet (i'm interested in CD19+ cells, but for now i use CD3+ as a control). I do know there are kits that are...
22 September 2019 3,210 3 View
thank you in advance!
11 August 2019 7,682 1 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
Hello: I am looking for articles that address e-commerce businesses and COVID-19 survivors, as well as Entrepreneurial strategies that reflect e-commerce businesses. Blessings Evelyn Dempsey
05 August 2024 5,332 3 View
How to enhanced h-index in Research Gate?
04 August 2024 3,368 4 View
Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...
02 August 2024 6,735 1 View
I am using a Bruker 600M solid-state NMR spectrometer with a Micro 2.5 microimaging system. The test sample is a tube of 1M LiCl aqueous solution, and the nucleus detected is 1H. I am trying to...
01 August 2024 9,227 1 View
We are looking for projects for a team of professional developers About us We are a team of experienced developers with over 10 years of experience in various programming fields. Our mission is to...
30 July 2024 8,377 0 View
I'm using a InVia Renishaw Raman microscope system (785nm wavelength, 600l/mm grating density) to acquire Raman images on human brain samples, at 50um resolution. The samples are placed on MgF2...
30 July 2024 8,771 3 View
Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...
29 July 2024 8,624 1 View