Athanasios Sachinidis

3 Questions 6 Answers 0 Followers

Questions related from Athanasios Sachinidis

Is there any human B cell line which serves as a model of autoimmunity??

I know it's not easy to find such a cell line (most of them are models of cancer afterall and it's also impossible for a cell line to produce autoantibodies), but I want to perform some in vitro...

11 January 2020 8,031 0 View

Has anyone successfully stained (intracellular staining) for T-bet with the Cytofix/Cytoperm Kit from BD Biosciences??

I isolate PBMCs from human blood and try to perform intracellular staining for T-bet (i'm interested in CD19+ cells, but for now i use CD3+ as a control). I do know there are kits that are...

23 September 2019 3,047 3 View

How to get rid of a negative control's Ct value in qPCR?

I run a negative control in qPCR, but I get a Ct value close to 25. I have already changed primers, ddH2O and even SYBR, but the problem still remains..

14 September 2017 8,381 6 View