Athanasios Sachinidis

6 Questions 11 Answers 0 Followers

Questions related from Athanasios Sachinidis

How much T-bet do B cells express in healthy humans??

I have some flow cytometry issues with T-bet staining, thus I would like to ask about the transcription factor's approximate percentage (a mean, actually ) in peripheral blood samples from healthy...

09 October 2022 2,391 2 View

Is there any human B cell line which serves as a model of autoimmunity??

I know it's not easy to find such a cell line (most of them are models of cancer afterall and it's also impossible for a cell line to produce autoantibodies), but I want to perform some in vitro...

11 January 2020 8,037 0 View

Has anyone successfully used the EasySep Human CD19 Positive Selection Kit II from STEMCELL Technologies??

I use human blood samples (~8ml each) from healthy individuals and perform centrifuge over lymphoprep, in order to isolate the PBMCs. Then, I use the EasySep Kit to isolate the CD19+ cells. The...

18 November 2019 8,864 3 View

Has anyone successfully stained (intracellular staining) for T-bet with the Cytofix/Cytoperm Kit from BD Biosciences??

I isolate PBMCs from human blood and try to perform intracellular staining for T-bet (i'm interested in CD19+ cells, but for now i use CD3+ as a control). I do know there are kits that are...

23 September 2019 3,080 3 View

How to get rid of a negative control's Ct value in qPCR?

I run a negative control in qPCR, but I get a Ct value close to 25. I have already changed primers, ddH2O and even SYBR, but the problem still remains..

14 September 2017 8,409 6 View

Unstable Expression of housekeeping gene (GAPDH)

Hi there, I'm treating MCF7 for an hour with LPS (1ug/ml) in order to enhance the mRNA levels of IL-6 . I measure the expression via Real Time PCR. The treatment seems to affect GAPDH levels...

05 August 2017 7,437 8 View