Athanasios Sachinidis

8 Questions 13 Answers 0 Followers

Questions related from Athanasios Sachinidis

How much T-bet do B cells express in healthy humans??

I have some flow cytometry issues with T-bet staining, thus I would like to ask about the transcription factor's approximate percentage (a mean, actually ) in peripheral blood samples from healthy...

09 October 2022 2,446 2 View

How to induce CD11c+ T-bet+ B cells in human PBMC cell cultures??

In human PBMC cell cultures, is it necessary to use anti-Ig (in conjunction with other stimuli, such as IFNγ, IL-21and/or R848) to induce CD11c+T-bet+ B cells?? The cytokines and the TLR7-ligand...

17 January 2022 1,296 1 View

Is there any human B cell line which serves as a model of autoimmunity??

I know it's not easy to find such a cell line (most of them are models of cancer afterall and it's also impossible for a cell line to produce autoantibodies), but I want to perform some in vitro...

11 January 2020 8,122 0 View

Has anyone successfully used the EasySep Human CD19 Positive Selection Kit II from STEMCELL Technologies??

I use human blood samples (~8ml each) from healthy individuals and perform centrifuge over lymphoprep, in order to isolate the PBMCs. Then, I use the EasySep Kit to isolate the CD19+ cells. The...

18 November 2019 8,921 3 View

Has anyone successfully stained (intracellular staining) for T-bet with the Cytofix/Cytoperm Kit from BD Biosciences??

I isolate PBMCs from human blood and try to perform intracellular staining for T-bet (i'm interested in CD19+ cells, but for now i use CD3+ as a control). I do know there are kits that are...

23 September 2019 3,238 3 View

How to get rid of a negative control's Ct value in qPCR?

I run a negative control in qPCR, but I get a Ct value close to 25. I have already changed primers, ddH2O and even SYBR, but the problem still remains..

14 September 2017 8,481 6 View

Unstable Expression of housekeeping gene (GAPDH)

Hi there, I'm treating MCF7 for an hour with LPS (1ug/ml) in order to enhance the mRNA levels of IL-6 . I measure the expression via Real Time PCR. The treatment seems to affect GAPDH levels...

05 August 2017 7,484 8 View

ScRNA seq data derived from PBMCs of SSc patients

Are there any scRNA-seq data, regarding the PBMCs of scleroderma/systemic sclerosis (SSc) patients? Thus far, I only found data derived from skin and/or lung biopsies ...! PS: I'm interested in B...

01 January 1970 8,264 2 View