How to evaluate how long dung is stable to act as a suitable substrate to coprophilous fungi growth?

Thanks for any contribution! Francisco.

08 September 2018 2,083 3 View

How to choose a good culture medium to grow fungi in laboratory?

Hi to all! How to choose a good culture medium to grow fungi in laboratory? I'd like to know which medium is recommended to growth of saprophytic fungi for isolation and enzymatic activity...

04 May 2018 3,989 22 View

How sensitive are the methods of estimating fungal richness?

Dear colleagues, herein some points that I would like some discussion and, if possible, some answers in regards to global fungal richness estimates: The question of how many species of Fungi...

01 February 2018 6,279 2 View

Why ITS and 18S rRNA to fungal diversity?

Dear all, please, I would like to know, if someone could to define it to me, why, thinking in molecular sense, was choosen the internal transcribed spacer (ITS) and 18S rRNA to access fungal...

31 December 2017 6,752 7 View

What do you think about use of Denaturing Gradient Gel Electrophoresis (DGGE)?

What do you think about use of Denaturing Gradient Gel Electrophoresis (DGGE) for evaluation of structure of fungal communities? I think that technic is a good tool for understanding of...

10 November 2017 6,683 2 View

I would like to know which primer systems is most indicated to discribe and characterize arbuscular mycorrhizal fungi (AMF) communities in soil?

Once studies (e.g. Kohout et al. 2014) have shown strong bias towards the Glomeraceae in the LSU and SSU primer systems and some differences in the composition of AMF communities based on the...

07 August 2016 3,487 24 View

Does anyone, here, studied ecological aspects of mycorrhizae in Cerrado soils of Brazil? Can you send me that paper?

05 June 2016 5,442 2 View

Where and how to publish in mycology?

01 January 1970 9,727 47 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...

28 February 2021 606 3 View