Dear all, please, I would like to know, if someone could to define it to me, why, thinking in molecular sense, was choosen the internal transcribed spacer (ITS) and 18S rRNA to access fungal diversity?
And why ITS-region is best to access total fungi (for example in soil) and 18S rRNA is most suitable to access arbuscular mycorrhizal fungi?
Thank you for your response!
Best regards,
F. Calaça
A good paper related is available at:
Data Nuclear ribosomal internal transcribed spacer (ITS) region a...
The ITS loci, specially ITS1 – ITS2 and ITS can provide very accurate data about the specific identification of the fungal species. The reason behind that may be lies on the nature of these loci in terms of their very high consensus sequences among fungal genera. Furthermore, some ITS loci provide more accurate data to the species level. The detective ability of ITS regions extend beyond fungal species to include other micro- as long as macro-organisms.
In fact, for one group of fungi - namely AMF, 18S is used by researchers specializing on AMF. Since so much of data is avaliable on AMF 18S I would suggest to follow their path. The database of AMF 18S data is called MaarjAM and it is at https://maarjam.botany.ut.ee/.
A good paper related is available at:
Data Nuclear ribosomal internal transcribed spacer (ITS) region a...
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