How to evaluate how long dung is stable to act as a suitable substrate to coprophilous fungi growth?

Thanks for any contribution! Francisco.

08 September 2018 2,083 3 View

How to choose a good culture medium to grow fungi in laboratory?

Hi to all! How to choose a good culture medium to grow fungi in laboratory? I'd like to know which medium is recommended to growth of saprophytic fungi for isolation and enzymatic activity...

04 May 2018 3,989 22 View

How sensitive are the methods of estimating fungal richness?

Dear colleagues, herein some points that I would like some discussion and, if possible, some answers in regards to global fungal richness estimates: The question of how many species of Fungi...

01 February 2018 6,279 2 View

Why ITS and 18S rRNA to fungal diversity?

Dear all, please, I would like to know, if someone could to define it to me, why, thinking in molecular sense, was choosen the internal transcribed spacer (ITS) and 18S rRNA to access fungal...

31 December 2017 6,752 7 View

What do you think about use of Denaturing Gradient Gel Electrophoresis (DGGE)?

What do you think about use of Denaturing Gradient Gel Electrophoresis (DGGE) for evaluation of structure of fungal communities? I think that technic is a good tool for understanding of...

10 November 2017 6,683 2 View

Saprophytic fungi in litter?

Which the most suitable primer set to applied in PCR technique to evaluate communities of saprophytic fungi that grow on standing litter? Do you know any paper about this?

10 November 2016 950 4 View

I would like to know which primer systems is most indicated to discribe and characterize arbuscular mycorrhizal fungi (AMF) communities in soil?

Once studies (e.g. Kohout et al. 2014) have shown strong bias towards the Glomeraceae in the LSU and SSU primer systems and some differences in the composition of AMF communities based on the...

07 August 2016 3,487 24 View

Where and how to publish in mycology?

01 January 1970 9,727 47 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

The Shannon's index is inversely proportional to Simpson's index (1-D)?

I'm having a problem with my bloxplot with Shannon's and Simpson's indexs. The value of Shannon for my Community 1 indicating higher diversity (in relation of the Community 2), but have lower...

26 February 2021 3,033 9 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

Is this the write procedure for acid digestion of soil for heavy metal analysis using AAS?

How to do acid digestion of soil sample with 3:1 aquaregia in an open vessel?

23 February 2021 3,053 7 View

Are there packages (R or Python) for simple models (bucket?) to assess soil water content?

Hi everyone. I am researching droguht effects on soil water drought. I am looking for already implemented functions in R or Python to estimate soil water content from precipitation,...

21 February 2021 2,084 1 View

Which type of loading is to be used in dynamic analysis of Machien foundation?

Hello Everyone, Hope you all are doing well. I'm trying to simulate a machine foundation in ABAQUS with a geo-cell reinforced soil bed. Im totally confused to which type of loading would be...

21 February 2021 4,388 3 View