Hi Sadia, It just means the sequence was deposited by someone, who like you, did not get the DNA from a cultured isolate, but was sequencing DNA from a community. Since these can be uninformative, there is a filter you can click when doing blast searches. If you look at the window where you load your sequence, there is a line that labeled "Exclude" there is a check box for "uncultured/environmental sample sequences". If you click this box the search will only return matches to sequences from isolates with an identification.

Dear Dr.Frank Burns,

Thank you so much for the informative answer.

Best Regards

It means that the best matches are to "uncultured lactobacillus" and other similar species. One note of caution: your DNA sequence is very, very short. I wouldn't make any firm conclusions about the identity of your bacteria without using a longer DNA sequence.

Dear Katie .Thanks for adding your answer .May be I can reamplify and sequence my samples again before making final conclusion.

Regards