I have some queries about basics of neuroscience...?

09 October 2015 4,230 2 View

How can CoCl2 mimic hypoxia?

11 December 2014 807 4 View

Why am I getting a nonspecific HIF-1alpha blot?

10 November 2014 4,862 5 View

How do you induce Hypoxia in cancer cell lines?

Hi, I want to induced hypoxia in cancer cell lines to check regulation of VEGF signaling components. So is there any simple way to induced hypoxia in breast cancer cell lines like HCT116, MCF7 or...

09 October 2014 4,765 7 View

How do you protect proteins from precipitation and degradation?

09 October 2014 690 14 View

How to identify Drosophila Mutant...?

I am searching literature about Drosophila mutant identification. can anyone suggest me books or papers related to Drosophila mutant identification and their maintenance...? I also need previous...

08 September 2014 8,103 4 View

How do you check Sensitivity of PCR ?

Hello, I want to know that how to check sensitivity of PCR ?

08 September 2014 193 8 View

Why are tumor suppressor proteins down regulated in cancer cells?

Why are tumor suppressor proteins down regulated in cancer cells even they are not mutated. Can some one explain signaling for this down regulation of tumor suppressor protein with any example or...

08 September 2014 4,767 4 View

Can anyone provide me a protocol for Fusarium oxysporum culture?

I want to culture Fusarium oxysporum. Can anyone provide me detailed protocol for Fusarium oxysporum culture?

02 March 2014 9,951 13 View

Does quantum mechanics fit to explain such brain functions as behaviour, neurodegeneration and consciousness?

Or is brain function a biological phenomenon? My other question is whether consciousness really exists in brain or is it a product of brain activity?

01 February 2014 8,695 46 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Could you please suggests a tutorial or paper or any other materials to learn multiple gene expression in TCGA and GTEx databases?

I would like to analyze multiple gene expressions in normal and cancer tissue by using the data in the TCGA and GTEx databases. Please suggest a tutorial or paper showing step by step analysis of...

23 February 2021 9,007 3 View

Are there any databases for gene co-expression or expression pattern clustering?

I am currently working on gene clustering based on co-expression pattern in mouse brain. The problem is I do not have some solid way to test my result. Are there any suggestions for databases...

22 February 2021 4,374 3 View

Which CpGs have a significant effect on gene expression?

It is known that methylated CpG around TSS decreases gene expression. However, It is difficult to know exactly which CpG has a greater impact on gene expression. Are there any databases known...

20 February 2021 5,634 3 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

Purification of proteins from baculovirus-infected insect cells?

18 February 2021 6,665 6 View

Found that there was a multiple band when amplifiying targeted gene ?

i few days ago amplified specific gene using PCR with specific primers, but there was a multiple band in electrophoregram i found. out of 10 sample amplified, there was multiple band and there was...

18 February 2021 5,131 5 View