Run-off-Transcription Assay ?

Manish Joshi @Manish_Joshi3

05 May 2013 1 8K Report

Hi,

What is the run off transcription assay ?

Can anyone provide me protocol for this assay ?

Evgeny Klimuk

Hi!

What kind of transcription do you study? You can find protocol of in vitro transcription assay with E.coli RNAP in paper:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3275717/

Regards,

Evgeny

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