How to purify Plasmid DNA after RNAse treatments and Restriction enzyme digestion for high yield Capped RNA Transcription ?

I tried phenol/chloroform purification method with 2 microgram DNA after RNAse and Restriction enzyme digestion but I am not getting enough quantity of plasmid DNA at the end. I am just getting...

25 September 2020 7,591 4 View

I am doing for Protein-ligand MD simulation using Gromacs but facing some problem during Ligand Topology step. How to resolve it ?

after running python3 cgenff_charmm2gmx_py3_nx2.py LIG lig_fix.mol2 lig.str charmm36-jul2020.ff I am getting following error: error in atomgroup.py: read_mol2_coor_only: no. of atoms in mol2 (44)...

30 August 2020 4,546 3 View

What is the mesh grid density requirements for LES WALE simulation in ANSYS CFX?

I am currently working on performing an LES WALE simulation to extract pressure data, to be later used for acoustic simulation. I have some information about the grid density requirements in terms...

27 April 2017 4,259 5 View

How to distinguish between TPA and higher order effects present beyond cubic Nonlinear term?

The open aperture z-scan profiles reveal saturable absorption response in all the four samples measured, though the results follow Two Photon absorption but is it possible that higher-order...

25 August 2016 1,039 4 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Analysis of CRD two factor using R studio ?

Hello everyone. I have conducted the research on post harvest longevity of tomato crop using plastic packaging and different doses of chemicals as two different factors. The design of experiment I...

28 February 2021 8,189 3 View

How, if at all, can R scripts/packages be used to perform cluster analyses and motif analyses on digital trace data?

02 February 2021 728 3 View

Unbalanced panel data fixed fir effects and industry-by-year fixed effects. Is plm package suitable? or lme in rstudio?

I am not sure if an unbalanced panel data set doesn't give me good results. Do I have to have all years of my panel data? How do I select the variables? I only select those where I have no missing...

30 January 2021 7,258 6 View

How to plot Random Forest model as raster prediction map in RStudio?

Output_raster

27 January 2021 7,974 3 View

Why is there no ANOVA output for a certain predictor variable in my GLM (in R)?

Configurations: Rstudio v1.3.1093 Packages: car, lme4, MuMIn, DescTools Model: myGLM

22 January 2021 5,284 6 View

Which statistical test to use for comparing percentages between multiple groups?

Hello everyone! I am looking for some statistical help! I am working in in vitro micropropagation and one of the aspects that I am studying is the rooting of plants in 10 different growing media....

14 January 2021 7,575 5 View

[SEM] Why RStudio and AMOS report different p-values (but same estimates)?

Hi everyone, I have conducted a SEM analysis in AMOS then replicated it in RStudio. While I obtained identical estimates and model fit statistics, the p-values differ between software programs...

25 December 2020 3,669 4 View

Is it rational to identify differentially expressed genes from tissues (in silico) and examine gene expression on the associated cell lines (wet lab)?

I am going to evaluate the gene expression levels in the MKN45 cell line (gastric cancer). Before that, I want to identify critical differentially expressed genes obtained from tissues available...

20 December 2020 3,774 6 View

Is all normalization for microarray the same?

What i know, microarray data has vsn, loess and quantile normalization . i am dealing with GEO microarray datasets where i have to find the differential expresssed genes for NPC. im planning on...

10 December 2020 1,496 3 View